Document Detail

Rapid and highly sensitive pathotyping of avian influenza A H5N1 virus by using real-time reverse transcription-PCR.
MedLine Citation:
PMID:  17182758     Owner:  NLM     Status:  MEDLINE    
Rapid typing of the pathogenicity of avian influenza A viruses (AIV) of subtypes H5 and H7 is crucial to initiate adequate protective measures preventing the spread of highly pathogenic AIV (HPAIV). Here, a new real-time reverse transcription-PCR assay which enables sensitive and specific detection and cleavage site analysis of HPAIV H5N1 of the Qinghai lineage is described.
Bernd Hoffmann; Timm Harder; Elke Starick; Klaus Depner; Ortrud Werner; Martin Beer
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Publication Detail:
Type:  Evaluation Studies; Journal Article     Date:  2006-12-20
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  45     ISSN:  0095-1137     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  2007 Feb 
Date Detail:
Created Date:  2007-02-05     Completed Date:  2007-03-29     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  600-3     Citation Subset:  IM    
Institute of Diagnostic Virology, D-17493 Greifswald-Insel Riems, Germany.
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MeSH Terms
Animals, Wild / virology
Base Sequence
Birds / virology
Chickens / virology
Hemagglutinin Glycoproteins, Influenza Virus / chemistry*,  genetics,  metabolism
Influenza A Virus, H5N1 Subtype / classification*,  genetics,  pathogenicity*
Influenza in Birds / epidemiology*,  prevention & control,  virology*
Molecular Sequence Data
Poultry Diseases / virology
Reverse Transcriptase Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Time Factors
Turkeys / virology
Reg. No./Substance:
0/Hemagglutinin Glycoproteins, Influenza Virus; 0/hemagglutinin, avian influenza A virus

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