| Rapid generation of human T cell hybridomas. | |
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MedLine Citation:
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PMID: 3874914 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Using the concept of a selectable surface marker we have generated screenable human T cell hybridomas within 4-7 days of fusion. OKT4+ T cell blasts were fused with MOLT4, an OKT4- T cell line, and OKT4+ fusion products were obtained by indirect rosetting technique. Supernatants of this heterogeneous population of hybrids were screened and those with activities of interest were subjected to cloning on soft agar and re-screening to dissect out various activities. Fusion efficiency, by staining, ranges from 6 to 80% depending on the state of activation of the T blasts and the growth phase of the MOLT4 line. This method allows for immortalization of T cell subpopulations, clones and malignant cells to study factor production and potentially messenger RNA for these factors. Additionally selectable surface markers may be used in human B cell and rodent:human fusions, avoiding the innate toxicity of various selection media. |
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Authors:
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L Mayer; S M Fu; H G Kunkel |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of immunological methods Volume: 81 ISSN: 0022-1759 ISO Abbreviation: J. Immunol. Methods Publication Date: 1985 Aug |
Date Detail:
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Created Date: 1985-09-11 Completed Date: 1985-09-11 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 1305440 Medline TA: J Immunol Methods Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 271-81 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, Surface
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analysis Cell Fusion Humans Hybridomas / cytology* Karyotyping Lymphokines / biosynthesis Rosette Formation Selection, Genetic T-Lymphocytes / cytology* |
| Grant Support | |
ID/Acronym/Agency:
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AI-10811/AI/NIAID NIH HHS; AI-19080/AI/NIAID NIH HHS; CA-34546/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Surface; 0/Lymphokines |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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