Document Detail


Rapid expansion of allospecific cytotoxic T cell clones using nonspecific feeder cell lines without further addition of exogenous IL2.
MedLine Citation:
PMID:  6208657     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The availability of large numbers of functionally defined cloned T cells would facilitate biochemical and molecular studies and might be a prerequisite for in vivo immunotherapy with cytotoxic lymphocytes. Exogenous interleukin-2 (IL-2) is generally considered to be the most important medium supplement for the growth of human T cells or cloned T cells. We have studied the role of particular feeder cells or stimulator cells, or both, on the expansion of a panel of cytotoxic and noncytotoxic human T cell clones. By using a combination of peripheral blood lymphocytes (PBL) and Epstein-Barr virus (EBV)-transformed B cell lines (B-LCL) as feeder cells in the presence of lectins, we were able to achieve continuous and rapid expansion of phenotypically different allospecific cytotoxic and noncytotoxic T cell clones even without (further) addition of exogenous IL-2. This culture system allows expansion of cloned cells for up to 60 generations within two months with full retention of cytolytic activity and the original target cell specificity, and without the obligatory addition of the original stimulator cell. It was found that, in principle, all combinations of EBV-transformed B cell lines (or even leukemia-derived B cell lines) and PBL feeder cells could serve the purpose, although some B cell lines gave better results than others. No evidence for HLA restriction between responder and feeder or between feeder cells was found, because cells could also be expanded with autologous feeder cells alone. The system appeared to be suitable for the expansion of various types of cytotoxic and non cytotoxic T cells, including cloned directed against major histocompatibility complex (MHC) class I or MHC class II antigens as well as MHC-nonrestricted activated killer (AK) clones.
Authors:
R J van de Griend; R L Bolhuis
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Transplantation     Volume:  38     ISSN:  0041-1337     ISO Abbreviation:  Transplantation     Publication Date:  1984 Oct 
Date Detail:
Created Date:  1984-11-26     Completed Date:  1984-11-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0132144     Medline TA:  Transplantation     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  401-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
B-Lymphocytes / immunology
Cell Communication
Cell Line
Clone Cells / classification,  immunology
Epitopes*
Growth Substances / physiology
Humans
Interleukin-2 / pharmacology*
Isoantigens / immunology*
Lymphocyte Activation
Lymphocyte Culture Test, Mixed / methods*
T-Lymphocytes, Cytotoxic / classification,  immunology*
Chemical
Reg. No./Substance:
0/Epitopes; 0/Growth Substances; 0/Interleukin-2; 0/Isoantigens

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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