| Rapid exercise-induced changes in PGC-1alpha mRNA and protein in human skeletal muscle. | |
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MedLine Citation:
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PMID: 18653753 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The mRNA of the nuclear coactivator peroxisome proliferator-activated receptor-gamma coactivator-1alpha (PGC-1alpha) increases during prolonged exercise and is influenced by carbohydrate availability. It is unknown if the increases in mRNA reflect the PGC-1alpha protein or if glycogen stores are an important regulator. Seven male subjects [23 +/- 1.3 yr old, maximum oxygen uptake (Vo(2 max)) 48.4 +/- 0.8 ml.kg(-1).min(-1)] exercised to exhaustion ( approximately 2 h) at 65% Vo(2 max) followed by ingestion of either a high-carbohydrate (HC) or low-carbohydrate (LC) diet (7 or 2.9 g.kg(-1).day(-1), respectively) for 52 h of recovery. Glycogen remained depressed in LC (P < 0.05) while returning to resting levels by 24 h in HC. PGC-1alpha mRNA increased both at exhaustion (3-fold) and 2 h later (6.2-fold) (P < 0.05) but returned to rest levels by 24 h. PGC-1alpha protein increased (P < 0.05) 23% at exhaustion and remained elevated for at least 24 h (P < 0.05). While there was no direct treatment effect (HC vs. LC) for PGC-1alpha mRNA or protein, there was a linear relationship between the changes in glycogen and those in PGC-1alpha protein during exercise and recovery (r = -0.68, P < 0.05). In contrast, PGC-1beta did not increase with exercise but rather decreased (P < 0.05) below rest level at 24 and 52 h, and the decrease was greater (P < 0.05) in LC. PGC-1alpha protein content increased in prolonged exercise and remained upregulated for 24 h, but this could not have been predicted by the changes in mRNA. The beta-isoform declined rather than increasing, and this was greater when glycogen was not resynthesized to rest levels. |
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Authors:
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Anila S Mathai; Arend Bonen; Carley R Benton; D L Robinson; Terry E Graham |
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Publication Detail:
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Type: Comparative Study; Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't Date: 2008-07-24 |
Journal Detail:
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Title: Journal of applied physiology (Bethesda, Md. : 1985) Volume: 105 ISSN: 8750-7587 ISO Abbreviation: J. Appl. Physiol. Publication Date: 2008 Oct |
Date Detail:
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Created Date: 2008-10-07 Completed Date: 2008-11-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8502536 Medline TA: J Appl Physiol Country: United States |
Other Details:
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Languages: eng Pagination: 1098-105 Citation Subset: IM |
Affiliation:
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Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Blood Glucose / metabolism Carrier Proteins / metabolism Cross-Over Studies Diet, Carbohydrate-Restricted* Dietary Carbohydrates / administration & dosage, metabolism* Exercise / physiology* Fatty Acids, Nonesterified / blood Glycogen / metabolism Heat-Shock Proteins / genetics, metabolism* Humans Insulin / blood Male Muscle Contraction* Muscle, Skeletal / metabolism* RNA, Messenger / metabolism* Recovery of Function Time Factors Transcription Factors / genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Blood Glucose; 0/Carrier Proteins; 0/Dietary Carbohydrates; 0/Fatty Acids, Nonesterified; 0/Heat-Shock Proteins; 0/PPARGC1A protein, human; 0/PPARGC1B protein, human; 0/RNA, Messenger; 0/Transcription Factors; 11061-68-0/Insulin; 9005-79-2/Glycogen |
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