| Rapid enrichment and detection of melanoma cells from peripheral blood mononuclear cells by a new assay combining immunomagnetic cell sorting and immunocytochemical staining. | |
| | |
MedLine Citation:
|
PMID: 11092033 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Commonly used methods for detection of melanoma cells in blood, including RT-PCR and immunocytochemistry, display only a limited sensitivity and specificity. Reliable detection of less than one melanoma cell per ml of blood is hardly possible using these methods. To obtain greater sensitivity so that a single melanoma cell in up to 25 ml of blood can be detected (5 x 10(7) peripheral blood mononuclear cells, or PBMC), we developed a new assay for combined enrichment and immunocytochemical detection of disseminated melanoma cells from PBMC of patients with malignant melanomas. Melanoma cells are directly magnetically labeled using colloidal superparamagnetic microparticles approximately 60 nm in diameter conjugated to the anti-melanoma monoclonal antibody 9.2.27, with no reactivity to normal cells in blood. Magnetically labeled melanoma cells are enriched from PBMC by magnetic cell separation and detected by a new approach for immunocytochemical staining with monoclonal mouse anti-melanoma antibodies (anti-MelanA and HMB-45). The efficiency of this assay was demonstrated in a model system in which 5-500 tumor cells from the melanoma cell line SK-MEL-28 were seeded into PBMC samples from healthy donors containing 5 x 10(7) leukocytes. Mean recovery of the seeded tumor cells was 47.4 +/- 13.99% (n = 15). Applying the assay to 20-50 ml blood samples of patients with stage III-IV malignant melanomas, we were able to detect melanoma cells in two of eight patients (25%). |
| | |
Authors:
|
C Siewert; M Herber; N Hunzelmann; O Fodstad; S Miltenyi; M Assenmacher; J Schmitz |
Related Documents
:
|
16192293 - An improved method for clearing and staining free-hand sections and whole-mount samples. 7542673 - Clear cell papulosis: report of three cases of a newly recognized disease. 14671613 - No correlation between the proliferative status of bcr-abl positive cell lines and the ... |
Publication Detail:
|
Type: Comparative Study; Journal Article |
Journal Detail:
|
Title: Recent results in cancer research. Fortschritte der Krebsforschung. Progrès dans les recherches sur le cancer Volume: 158 ISSN: 0080-0015 ISO Abbreviation: Recent Results Cancer Res. Publication Date: 2001 |
Date Detail:
|
Created Date: 2001-02-14 Completed Date: 2001-03-15 Revised Date: 2008-11-21 |
Medline Journal Info:
|
Nlm Unique ID: 0044671 Medline TA: Recent Results Cancer Res Country: GERMANY |
Other Details:
|
Languages: eng Pagination: 51-60 Citation Subset: IM |
Affiliation:
|
Miltenyi Biotec GmbH, Bergisch Gladbach, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Antibodies, Monoclonal Antigens, Neoplasm Humans Immunohistochemistry / methods* Immunomagnetic Separation / methods* Leukocytes, Mononuclear / pathology* Melanoma / diagnosis* Neoplasm Metastasis / diagnosis Neoplasm Proteins / analysis Neoplasm Staging Neoplastic Cells, Circulating / pathology* Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
|
0/Antibodies, Monoclonal; 0/Antigens, Neoplasm; 0/MART1 antigen; 0/Neoplasm Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Immunobead-based detection and characterization of circulating tumor cells in melanoma patients.
Next Document: Reverse transcriptase polymerase chain reaction (RT-PCR) detection of melanoma-related transcripts i...