Document Detail

Rapid effects of 17beta-estradiol on TRPV5 epithelial Ca2+ channels in rat renal cells.
MedLine Citation:
PMID:  19463684     Owner:  NLM     Status:  MEDLINE    
The renal distal tubules and collecting ducts play a key role in the control of electrolyte and fluid homeostasis. The discovery of highly calcium selective channels, Transient Receptor Potential Vanilloid 5 (TRPV5) of the TRP superfamily, has clarified the nature of the calcium entry channels. It has been proposed that this channel mediates the critical Ca(2+) entry step in transcellular Ca(2+) re-absorption in the kidney. The regulation of transmembrane Ca(2+) flux through TRPV5 is of particular importance for whole body calcium homeostasis.In this study, we provide evidence that the TRPV5 channel is present in rat cortical collecting duct (RCCD(2)) cells at mRNA and protein levels. We demonstrate that 17beta-estradiol (E(2)) is involved in the regulation of Ca(2+) influx in these cells via the epithelial Ca(2+) channels TRPV5. By combining whole-cell patch-clamp and Ca(2+)-imaging techniques, we have characterized the electrophysiological properties of the TRPV5 channel and showed that treatment with 20-50nM E(2) rapidly (<5min) induced a transient increase in inward whole-cell currents and intracellular Ca(2+) via TRPV5 channels. This rise was significantly prevented when cells were pre-treated with ruthenium red and completely abolished in cells treated with siRNA specifically targeting TRPV5.These data demonstrate for the first time, a novel rapid modulation of endogenously expressed TRPV5 channels by E(2) in kidney cells. Furthermore, the results suggest calcitropic effects of E(2). The results are discussed in relation to present concepts of non-genomic actions of E(2) in Ca(2+) homeostasis.
Mustapha Irnaten; Nicolas Blanchard-Gutton; Jeppe Praetorius; Brian J Harvey
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-02-21
Journal Detail:
Title:  Steroids     Volume:  74     ISSN:  1878-5867     ISO Abbreviation:  Steroids     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-05-25     Completed Date:  2009-09-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0404536     Medline TA:  Steroids     Country:  United States    
Other Details:
Languages:  eng     Pagination:  642-9     Citation Subset:  IM    
Molecular Medicine Laboratories, Royal College of Surgeons in Ireland, Beaumont Hospital, PO Box 9063, Dublin 9, Ireland.
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MeSH Terms
Calcium / metabolism
Calcium Channels / genetics,  metabolism*
Cell Line
Electrophysiological Processes / drug effects
Estradiol / pharmacology*
Gene Expression Regulation / drug effects
Gene Knockdown Techniques
Intracellular Space / drug effects,  metabolism
Kidney / cytology*,  drug effects*,  metabolism,  physiology
Patch-Clamp Techniques
RNA, Small Interfering / genetics
Ruthenium Red / metabolism
TRPV Cation Channels / genetics,  metabolism*
Time Factors
Reg. No./Substance:
0/Calcium Channels; 0/Ecac1 protein, rat; 0/RNA, Small Interfering; 0/TRPV Cation Channels; 11103-72-3/Ruthenium Red; 50-28-2/Estradiol; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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