Document Detail


Rapid drug extraction from human whole blood using a modified QuEChERS extraction method.
MedLine Citation:
PMID:  22682428     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
A modified QuEChERS (quick, easy, cheap, effective, rugged and safe) extraction method followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been developed for the simultaneous determination of forensically important drugs and poisons (more than 90 compounds) in human whole blood. Because the QuEChERS method is commonly used for the analysis of pesticide residues in foods, we customized the QuEChERS method for forensic use. This extraction method consists essentially of two steps: (1) extraction/partitioning and (2) dispersive-solid phase extraction. In step 1, three-fold diluted blood was mixed with an internal standard (D(5)-diazepam for basic drugs or D(5)-phenobarbital for acidic drugs) solution, a QuEChERS pre-packed extraction kit (containing magnesium sulfate and sodium acetate) and a stainless steel bead, then partitioned into three layers by centrifugation. In step 2, the top layer (acetonitrile) was transferred into a centrifuge tube containing a dispersive-solid phase sorbent (containing primary secondary amine, end-capped octadecylsilane, and magnesium sulfate) and mixed for purification. After the centrifugation, supernatant was injected into LC-MS/MS. The QuEChERS method was applied in an autopsy case and we confirmed that this method can easily extract various types of drugs and metabolites from human whole blood. The combination of the modified QuEChERS method and LC-MS/MS could enable technicians inexperienced in forensic toxicological analysis to acquire reliable data quickly and easily.
Authors:
Kiyotaka Usui; Yoshie Hayashizaki; Masaki Hashiyada; Masato Funayama
Related Documents :
22608428 - Determination of polycyclic aromatic hydrocarbons in water samples using online microex...
939228 - A comparative study of the bessey method and hausamen method for serum alkaline phospha...
22298288 - Polar aprotic modifiers for chromatographic separation and back-exchange reduction for ...
3137308 - Enzymatic colorimetric method for the determination of inorganic phosphorus in serum an...
9187388 - Simultaneous determination of midazolam and its metabolites 1-hydroxymidazolam and 4-hy...
22608428 - Determination of polycyclic aromatic hydrocarbons in water samples using online microex...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-6
Journal Detail:
Title:  Legal medicine (Tokyo, Japan)     Volume:  -     ISSN:  1873-4162     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-6-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100889186     Medline TA:  Leg Med (Tokyo)     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.
Affiliation:
Division of Forensic Medicine, Department of Public Health and Forensic Medicine, Tohoku University Graduate School of Medicine, Sendai, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  An autopsy case of sudden unexpected nocturnal death syndrome with R1193Q polymorphism in the SCN5A ...
Next Document:  Do prescription stimulants increase the risk of adverse cardiovascular events?: A systematic review.