Document Detail


Rapid and direct staphylocoagulase assay that uses a chromogenic substrate for identification of Staphylococcus aureus.
MedLine Citation:
PMID:  7309847     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A rapid and direct assay of staphylocoagulase that uses chromogenic substrate Chromozym TH was developed for the routine identification of Staphylococcus aureus. False-positive and false-negative reactions were eliminated by measuring the activity of staphylocoagulase-prothrombin complex directly. The method was compared with three clotting assays. The results show that the new assay is specific, quantitative, and easily automated. If offers clinical laboratories a more reliable assay of staphylocoagulase and is a valuable aid for the rapid identification of S. aureus.
Authors:
W Engels; M A Kamps; C P van Boven
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  14     ISSN:  0095-1137     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  1981 Nov 
Date Detail:
Created Date:  1982-02-12     Completed Date:  1982-02-12     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  496-500     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Blood Coagulation
Coagulase / metabolism*
Culture Media
False Positive Reactions
Methods
Oligopeptides / metabolism
Prothrombin / metabolism
Serum Albumin, Bovine
Staphylococcus aureus / classification*,  enzymology
Chemical
Reg. No./Substance:
0/Coagulase; 0/Culture Media; 0/Oligopeptides; 0/Serum Albumin, Bovine; 61876-61-7/chromozym TH; 9001-26-7/Prothrombin
Comments/Corrections

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