Document Detail


Rapid, direct fluorescent-antibody method for the detection of salmonellae in food and feeds.
MedLine Citation:
PMID:  4564047     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An improved immunofluorescent-antibody (FA) method for the detection of salmonellae in foods and feeds was developed. This FA method combines a rapid cultural phase and a serological phase that allow for propagation of salmonellae in a minimum time, employing the industrial 8-hr work day as a guide. Two hundred fifty naturally contaminated human food and animal feed samples, representing 647 trials, were tested by the FA method. A total of 18 different food and feed samples was used. The method used by the Association of Official Analytical Chemists (AOAC) for the detection of salmonellae was the control method. The percent agreement when comparing the FA slide method to the AOAC method ranged from 87.1 to 95.3%, depending upon the conjugated antisera used in comparative studies.
Authors:
N F Insalata; C W Mahnke; W G Dunlap
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Applied microbiology     Volume:  24     ISSN:  0003-6919     ISO Abbreviation:  Appl Microbiol     Publication Date:  1972 Oct 
Date Detail:
Created Date:  1973-01-18     Completed Date:  1973-01-18     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7605802     Medline TA:  Appl Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  645-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animal Feed*
Antigens, Bacterial / analysis
Culture Media
Evaluation Studies as Topic
Fluorescent Antibody Technique*
Food Contamination
Food Microbiology*
Immune Sera
Methods
Microscopy, Fluorescence
Salmonella / immunology,  isolation & purification*
Salmonella typhimurium / immunology,  isolation & purification
Time Factors
Chemical
Reg. No./Substance:
0/Antigens, Bacterial; 0/Culture Media; 0/Immune Sera
Comments/Corrections

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