| Rapid, direct fluorescent-antibody method for the detection of salmonellae in food and feeds. | |
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MedLine Citation:
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PMID: 4564047 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An improved immunofluorescent-antibody (FA) method for the detection of salmonellae in foods and feeds was developed. This FA method combines a rapid cultural phase and a serological phase that allow for propagation of salmonellae in a minimum time, employing the industrial 8-hr work day as a guide. Two hundred fifty naturally contaminated human food and animal feed samples, representing 647 trials, were tested by the FA method. A total of 18 different food and feed samples was used. The method used by the Association of Official Analytical Chemists (AOAC) for the detection of salmonellae was the control method. The percent agreement when comparing the FA slide method to the AOAC method ranged from 87.1 to 95.3%, depending upon the conjugated antisera used in comparative studies. |
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Authors:
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N F Insalata; C W Mahnke; W G Dunlap |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Applied microbiology Volume: 24 ISSN: 0003-6919 ISO Abbreviation: Appl Microbiol Publication Date: 1972 Oct |
Date Detail:
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Created Date: 1973-01-18 Completed Date: 1973-01-18 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7605802 Medline TA: Appl Microbiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 645-9 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animal Feed* Antigens, Bacterial / analysis Culture Media Evaluation Studies as Topic Fluorescent Antibody Technique* Food Contamination Food Microbiology* Immune Sera Methods Microscopy, Fluorescence Salmonella / immunology, isolation & purification* Salmonella typhimurium / immunology, isolation & purification Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Bacterial; 0/Culture Media; 0/Immune Sera |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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