Document Detail

Rapid diagnosis of Helicobacter pylori infection in gastric imprint smears.
MedLine Citation:
PMID:  15689086     Owner:  NLM     Status:  MEDLINE    
The objective of this study was to determine the sensitivity, specificity, positive (PPV), and negative predictive values (NPV) of Diff-Quik-stained gastric imprint cytology smears in the detection of H. pylori compared with histology. Air-dried imprint smears of gastric biopsies from 150 patients were stained by the Diff-Quik method in the endoscopy suite and examined for H. pylori, providing results within minutes. The presence of inflammation and intestinal metaplasia were documented. The same biopsy was processed and stained with H&E and Warthin-Starry stains, and reviewed by a different pathologist blind to the imprint cytology results. Ninety-four of the 150 patients were male with a mean age of 50 years. Based on histology, the H. pylori prevalence was very low at 8%. The sensitivity and specificity of imprint cytology in the detection of H. pylori were 83.3% and 100%, respectively. The PPV and NPV were 100% and 98.6%, respectively. There were two false negatives and no false positives. A combination of imprint cytology and histology achieved 100% sensitivity. Imprint smears did not provide added value over histology with regards to inflammation and metaplasia. Gastric imprint smears stained with Diff-Quik method is a rapid, cheap, and reliable method for the detection of H. pylori and have their best results when complemented with histology.
Gurjeet Kaur; Manoharan Madhavan; Amir Hakim Basri; Abdul Hamid Mat Sain; Mohd Shaiful Bahrun Hussain; Mohd Kamal Yatiban; Nyi Nyi Naing
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Validation Studies    
Journal Detail:
Title:  The Southeast Asian journal of tropical medicine and public health     Volume:  35     ISSN:  0125-1562     ISO Abbreviation:  Southeast Asian J. Trop. Med. Public Health     Publication Date:  2004 Sep 
Date Detail:
Created Date:  2005-02-03     Completed Date:  2005-05-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0266303     Medline TA:  Southeast Asian J Trop Med Public Health     Country:  Thailand    
Other Details:
Languages:  eng     Pagination:  676-80     Citation Subset:  IM    
Department of Pathology, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia.
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MeSH Terms
Aged, 80 and over
Azure Stains / diagnostic use
Cytodiagnosis / methods*
Endoscopy, Digestive System
Helicobacter Infections / pathology*
Helicobacter pylori / isolation & purification*
Methylene Blue / diagnostic use
Middle Aged
Predictive Value of Tests
Pyloric Antrum / microbiology*,  pathology
Staining and Labeling / methods*
Xanthenes / diagnostic use
Reg. No./Substance:
0/Azure Stains; 0/Diff Quik; 0/Xanthenes; 61-73-4/Methylene Blue

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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