Document Detail

Rapid determination of trans-fatty acids in human adipose tissue. Comparison of attenuated total reflection infrared spectroscopy and gas chromatography.
MedLine Citation:
PMID:  9521553     Owner:  NLM     Status:  MEDLINE    
A rapid attenuated total reflection (ATR) infrared (IR) spectroscopy procedure was used for quantitating the levels of total trans-fatty acid methyl ester (FAME) derivatives in neat (without solvent) test samples isolated from human adipose tissue. This procedure requires no weighing of the laboratory sample. The single-beam spectrum of the trans-containing FAMEs was 'ratioed' against that of a reference material having only cis double bonds in order to obtain a symmetric absorption band at 966 cm(-1) on a horizontal background. A single-reflection ATR diamond cell that requires only about 1 microl of neat FAMEs was used. The average level of trans-fatty acids in human adipose tissue found by ATR (3.07+/-0.27%) was generally higher than that obtained by gas chromatography (2.59+/-0.20%). Reasons for such a difference are discussed.
J Fritsche; H Steinhart; M M Mossoba; M P Yurawecz; N Sehat; Y Ku
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of chromatography. B, Biomedical sciences and applications     Volume:  705     ISSN:  1387-2273     ISO Abbreviation:  J. Chromatogr. B Biomed. Sci. Appl.     Publication Date:  1998 Feb 
Date Detail:
Created Date:  1998-05-13     Completed Date:  1998-05-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9714109     Medline TA:  J Chromatogr B Biomed Sci Appl     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  177-82     Citation Subset:  IM    
University of Hamburg, Institute of Biochemistry and Food Chemistry, Germany.
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MeSH Terms
Adipose Tissue / chemistry*
Child, Preschool
Chromatography, Gas*
Fatty Acids / analysis
Fatty Acids, Unsaturated / analysis*
Reproducibility of Results
Sensitivity and Specificity
Spectrophotometry, Infrared* / methods
Reg. No./Substance:
0/Fatty Acids; 0/Fatty Acids, Unsaturated

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