Document Detail


Rapid determination of six urinary benzene metabolites in occupationally exposed and unexposed subjects.
MedLine Citation:
PMID:  15051535     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A gas chromatography-mass spectrometry method for measurement of the main urinary metabolites of benzene, namely, phenol, catechol, hydroquinone, 1,2,4-trihydroxybenzene (trihydroxybenzene), t,t-muconic acid (muconic acid), and S-phenylmercapturic acid (phenylmercapturic acid), is reported. The method is considerably simpler than existing assays. It was applied to urine from benzene-exposed subjects and controls from Shanghai, China. When subjects were divided into controls (n = 44), those exposed to </= 31 ppm benzene (n = 21), and those exposed to > 31 ppm benzene (n = 19), Spearman correlations with exposure category were >/= 0.728 (p < 0.0001) for all metabolites except trihydroxybenzene. When exposed subjects were compared on an individual basis, all metabolites, including trihydroxybenzene, were significantly correlated with benzene exposure (Pearson r >/= 0.472, p </= 0.002) and with each other (Pearson r >/= 0.708, p < 0.0001). Ratios of individual metabolite levels to total metabolite levels provided evidence of competitive inhibition of CYP 2E1 enzymes leading to increased production of phenol, catechol, and phenylmercapturic acid at the expense of hydroquinone, trihydroxybenzene, and muconic acid. Since all metabolites were detected in all control subjects, the method can be applied to persons exposed to environmental levels of benzene.
Authors:
Suramya Waidyanatha; Nathaniel Rothman; Guilan Li; Martyn T Smith; Songnian Yin; Stephen M Rappaport
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Analytical biochemistry     Volume:  327     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  2004 Apr 
Date Detail:
Created Date:  2004-03-30     Completed Date:  2004-11-29     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  184-99     Citation Subset:  IM    
Affiliation:
Department of Environmental Sciences and Engineering, School of Public Health, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7431, USA.
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MeSH Terms
Descriptor/Qualifier:
Air Pollutants / metabolism,  urine*
Benzene / metabolism*
Benzene Derivatives / chemistry,  urine
Gas Chromatography-Mass Spectrometry / methods*
Humans
Industry
Molecular Structure
Occupational Exposure*
Grant Support
ID/Acronym/Agency:
P30ES10126/ES/NIEHS NIH HHS; P42ES04705/ES/NIEHS NIH HHS; P42ES05948/ES/NIEHS NIH HHS; P50ES01896/ES/NIEHS NIH HHS; R01ES06721/ES/NIEHS NIH HHS
Chemical
Reg. No./Substance:
0/Air Pollutants; 0/Benzene Derivatives; 71-43-2/Benzene

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