Document Detail

Rapid determination of orotic acid in urine by a fast liquid chromatography/tandem mass spectrometric method.
MedLine Citation:
PMID:  12672132     Owner:  NLM     Status:  MEDLINE    
Quantification of orotic acid (uracil-6-carboxylic acid) in urine is an important tool to diagnose some inherited diseases, such as urea cycle disorder (OTCD) and hereditary orotic aciduria. New rapid analytical methods are necessary to provide high-throughput orotic acid analyses. A new analytical method has been developed for the rapid analysis of orotic acid in urine by liquid chromatography coupled with ion spray tandem mass spectrometry (LC/MS/MS). After a sample dilution 1:20, the analysis was performed in the selected reaction monitoring mode in which orotic acid was detected through the transition m/z 155 to 111. The retention time was 3.9 min in a 4.5-min analysis. Daily calibration between 0.5-5.0 micromol/L of orotic acid, corresponding to 10-100 micromol/L in urine before the 1:20 dilution, offered consistent linearity and reproducibility. Interassay coefficient of variance (c.v.) was 4.97% at a mean concentration of 10.99 micromol/L. The sensitivity and specificity of tandem mass spectrometry permitted a high volume of analyses of orotic acid. The sample preparation is simple, inexpensive and not time demanding.
Giancarlo la Marca; Bruno Casetta; Enrico Zammarchi
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Rapid communications in mass spectrometry : RCM     Volume:  17     ISSN:  0951-4198     ISO Abbreviation:  Rapid Commun. Mass Spectrom.     Publication Date:  2003  
Date Detail:
Created Date:  2003-04-02     Completed Date:  2003-05-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8802365     Medline TA:  Rapid Commun Mass Spectrom     Country:  England    
Other Details:
Languages:  eng     Pagination:  788-93     Citation Subset:  IM    
Copyright Information:
Copyright 2003 John Wiley & Sons, Ltd.
Metabolic and Neuromuscolar Unit, 1st Pediatric Clinic, A Meyer Children's Hospital, University of Florence, 50132 Florence, Italy.
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MeSH Terms
Chromatography, Liquid / methods*
Creatinine / urine
Mass Spectrometry / methods*
Molecular Structure
Orotic Acid / chemistry,  urine*
Reproducibility of Results
Sensitivity and Specificity
Time Factors
Reg. No./Substance:
60-27-5/Creatinine; 65-86-1/Orotic Acid

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