| Rapid determination of indomethacin and salicylic acid in serum by means of reversed-phase liquid chromatography. | |
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MedLine Citation:
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PMID: 7391154 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A method for the quantitative analysis of indomethacin and salicylic acid in blood serum and urine by high-performance liquid chromatography is described. A C18-bonded silica was used as the stationary phase and mixtures of ethanol, n-butanol and aqueous buffer as the mobile phase. Before injection the serum is deproteinized and extracted in one step. The recovery of the extraction was found to be 88% and 77% for indomethacin and salicylic acid, respectively. The relative standard deviations of the analysis for 0.5 micrograms indomethacin and 5 micrograms salicyclic acid per ml serum were 3.6% and 3.2%, respectively. The detection limits for indomethacin and salicylic acid were 2 ng. This corresponds for both substances to 0.1 micrograms/ml serum for an injection volume of 100 microliters. The method enables simultaneous determination of possibly formed metabolites. A number of concurrently administered drugs do not interfere with the analysis. The interactive effects of co-medication of indomethacin and salicylic acid on the serum concentration of indomethacin is demonstrated by measuring the pharmacokinetic curves. |
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Authors:
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C P Terweij-Groen; S Heemstra; J C Kraak |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of chromatography Volume: 181 ISSN: 0021-9673 ISO Abbreviation: J. Chromatogr. Publication Date: 1980 Mar |
Date Detail:
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Created Date: 1980-09-23 Completed Date: 1980-09-23 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0427043 Medline TA: J Chromatogr Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 385-97 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Arthritis, Rheumatoid
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blood,
drug therapy Chromatography, Liquid / methods Drug Interactions Humans Hydrogen-Ion Concentration Indomethacin / blood*, therapeutic use, urine Kinetics Salicylic Acids / blood*, therapeutic use, urine |
| Chemical | |
Reg. No./Substance:
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0/Salicylic Acids; 53-86-1/Indomethacin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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