| Rapid determination of diazepam and nordiazepam in plasma by electron capture gas--liquid chromatography. Application in clinical pharmacokinetic studies. | |
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MedLine Citation:
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PMID: 334786 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A rapid method was developed for the determination of diazepam and nordiazepam (N-desmethyldiazepam) in human plasma using electron capture gas--liquid chromatography (GLC--ECE). The concentration of diazepam and nordiazepam is determined using 0.5 ml of plasma extracted with 1.0 ml of benzene containing 25 ng/ml of methylnitrazepam as the internal standard. The benzene extract is removed and an aliquot is subjected to automated GLC-ECD analysis. The method has a sensitivity limit of 5 ng diazepam and 10ng nordiazepam per milliliter of plasma. The method was used to determine the plasma levels in man following the first 5-mg diazepam dose, as well as during chronic oral administration of 5 mg diazepam three times daily and 15 mg diazepam once a day. |
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Authors:
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R E Weinfeld; H N Posmanter; K C Khoo; C V Puglisi |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of chromatography Volume: 143 ISSN: 0021-9673 ISO Abbreviation: J. Chromatogr. Publication Date: 1977 Nov |
Date Detail:
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Created Date: 1977-12-29 Completed Date: 1977-12-29 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0427043 Medline TA: J Chromatogr Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 581-95 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Autoanalysis Chromatography, Gas Chromatography, Liquid Diazepam / administration & dosage, analogs & derivatives*, blood* Humans Nordazepam / administration & dosage, blood* |
| Chemical | |
Reg. No./Substance:
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1088-11-5/Nordazepam; 439-14-5/Diazepam |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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