Document Detail

Rapid determination of chlormequat in meat by dispersive solid-phase extraction and hydrophilic interaction liquid chromatography (HILIC)-electrospray tandem mass spectrometry.
MedLine Citation:
PMID:  22686367     Owner:  NLM     Status:  Publisher    
Abstraction: A rapid method for analyzing trace level of chlormequat (CQ) in meat samples by hydrophilic interaction liquid chromatography-electrospray tandem mass spectrometry was developed. The samples were extracted with acetonitrile, followed by a rapid clean-up through dispersive solid-phase extraction (DSPE) technique with octadecyl (C18) dispersive-SPE sorbents. The chromatographic separation was within 6 min based on hydrophilic interaction liquid chromatography (HILIC) column with 10 mM ammonium acetate and 0.1 % (v/v) formic acid in water/acetonitrile (v/v, 40:60) as the mobile phase. And quantification was performed using a matrix-matched calibration curve which was linear in the range of the 0.05-100 μg/L. The limit of detection (LOD) was estimated at 0.03 μg/kg for CQ based on a peak to peak signal-noise (S/N=3). The limit of quantification (LOQ) was 0.1 μg/kg based on the lowest spiked concentration with suitable precision and accuracy. The average recovery of CQ in spiked meat samples was 86.4-94.7 % at 2, 20 and 200 μg/kg. Finally, this method was applied to determine CQ in the livestock and poultry meats purchased from markets in Beijing in 2011. CQ was detected in all 12 samples and the concentration was 0.4-636.0 μg/kg. Concentrations in a chicken sample (636.0 μg/kg) and a goat meat sample (486.0 μg/kg) were founded to be 15.9 and 2.43 times of the corresponding Codex maximum residue limits, respectively.
Chunmei Li; Fen Jin; Zhiyong Yu; Yamei Qi; Xiaomei Shi; Miao Wang; Hua Shao; Maojun Jin; Jing Wang; Mingqi Yang
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-11
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  -     ISSN:  1520-5118     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-6-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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