Document Detail

Rapid determination of N,N-diethyl-m-toluamide and permethrin in human plasma by gas chromatography-mass spectrometry and pyridostigmine bromide by high-performance liquid chromatography.
MedLine Citation:
PMID:  16620527     Owner:  NLM     Status:  MEDLINE    
A rapid and highly sensitive gas chromatography-mass spectrometry (GC-MS) method for simultaneous determination of N,N-diethyl-m-toluamide (DEET) and permethrin with (2)H(10)-phenanthrene (98 atom %) as an internal standard and a separate external standard high-performance liquid chromatography (HPLC) method for pyridostigmine bromide (PB) determination in human plasma were developed and validated. The GC-MS method for DEET and permethrin quantification utilizes a one-step extraction with tert-butylmethylether. The HPLC method for PB quantification involves a solid-phase extraction and UV detection. The range of the analytical method for DEET and permethrin was 1 ng/mL to 100 ng/mL and for PB was 5 ng/mL to 100 ng/mL. Recovery from plasma proved to be more than 80%. The intraday precision ranged from 1.3% to 8% for DEET, from 2.1% to 11.4% for permethrin, and from 3.0% to 4.8% for PB. The interday precision was 3% for DEET, ranged from 5% to 9% for permethrin, and from 5% to 9% for PB. The accuracy for the limit of quantification was 92% +/- 8% relative standard deviation (RSD) for DEET, 112% +/- 11% RSD for permethrin, and 109% +/- 5% RSD for PB. All 3 compounds were stable in human plasma at -80 degrees C for at least 12 months and after 2 freeze-thaw cycles with RSD values ranging from 7.1% (DEET, 80 ng/mL) to 8.1% (DEET, 8 ng/mL), from 2.3% (permethrin, 80 ng/mL) to 11.6 % (permethrin, 8 ng/mL), and from 0.2% (PB, 80 ng/mL) to 3.6% (PB, 8 ng/mL). Both methods were successfully applied to pharmacokinetic/ pharmacodynamic studies of combined exposure of DEET (skin application), permethrin (treated uniforms), and PB (30 mg orally three times/day for four doses) in healthy volunteers (n = 81).
Svetlana A Cherstniakova; Gregory E Garcia; John Strong; Daoqin Bi; Julie Weitz; Michael J Roy; Louis R Cantilena
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of analytical toxicology     Volume:  30     ISSN:  0146-4760     ISO Abbreviation:  J Anal Toxicol     Publication Date:    2006 Jan-Feb
Date Detail:
Created Date:  2006-04-19     Completed Date:  2006-05-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7705085     Medline TA:  J Anal Toxicol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  21-6     Citation Subset:  IM    
Division of Clinical Pharmacology and Medical Toxicology, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814, USA.
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MeSH Terms
Chromatography, High Pressure Liquid*
DEET / blood*,  pharmacokinetics
Drug Stability
Gas Chromatography-Mass Spectrometry / methods*
Insect Repellents / blood*,  pharmacokinetics
Insecticides / blood*,  pharmacokinetics
Military Medicine
Permethrin / blood*,  pharmacokinetics
Pyridostigmine Bromide / blood*,  pharmacokinetics
Reproducibility of Results
Reg. No./Substance:
0/Insect Repellents; 0/Insecticides; 101-26-8/Pyridostigmine Bromide; 134-62-3/DEET; 52645-53-1/Permethrin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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