| Rapid detection of ricin in milk using immunomagnetic separation combined with surface-enhanced Raman spectroscopy. | |
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MedLine Citation:
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PMID: 22417440 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Ricin is a potential bioterrisiom agent. There is a critical need for a method that can rapidly and simply detect ricin and other bioterrisiom agents in complex food matrices such as milk. In this study, we demonstrated a rapid method that combined immunomagnetic separation (IMS) and surface-enhanced Raman spectroscopy (SERS) to detect ricin in whole milk. IMS was used to specifically capture the ricin out of the milk. Then, SERS was applied to analyze the IMS eluate mixed with silver dendrite nanosubstrates. This approach facilitated detection and quantification down to 4 μg/mL ricin in milk within 20 min, based on the results of principal component analysis and partial least squares analysis. The feasibility of using a portable Raman instrument shows great promise for on-site detection in a processing facility. Practical Application: The method described in this manuscript that combined IMS and SERS could be used for rapid detection of ricin and other protein toxins in complex food matrices such as milk within 20 min. The use of a portable Raman could facilitate the on-site detection in a processing facility. |
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Authors:
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Lili He; Bronwyn Deen; Tom Rodda; Ian Ronningen; Tim Blasius; Christy Haynes; Francisco Diez-Gonzalez; Theodore P Labuza |
Publication Detail:
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Type: Journal Article Date: 2011-05-10 |
Journal Detail:
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Title: Journal of food science Volume: 76 ISSN: 1750-3841 ISO Abbreviation: J. Food Sci. Publication Date: 2011 Jun |
Date Detail:
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Created Date: 2012-03-15 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0014052 Medline TA: J Food Sci Country: United States |
Other Details:
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Languages: eng Pagination: N49-53 Citation Subset: IM |
Copyright Information:
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© 2011 Institute of Food Technologists® |
Affiliation:
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Authors He, Deen, Rodda, Ronningen, Blasius, Diez-Gonzalez and Labuza are with Dept. of Food Science and Nutrition, Univ. of Minnesota, 1334 Eckles Ave., St. Paul, MN 55108, U.S.A. Author Haynes is with Dept. of Chemistry, Univ. of Minnesota, 207 Pleasant St. SE, Minneapolis, MN 55455, U.S.A. Direct inquiries to author Labuza (E-mail: tplabuza@umn.edu). |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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