| Rapid detection of the poly-gamma-D-glutamic acid capsular antigen of Bacillus anthracis by latex agglutination. | |
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MedLine Citation:
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PMID: 19345041 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Latex agglutination has been used to detect capsular polysaccharides from a variety of bacteria in body fluids. A latex agglutination assay was constructed for detection of the poly-gamma-D-glutamic acid (gammaDPGA) capsular polypeptide of Bacillus anthracis in serum from animal models of pulmonary anthrax. The assay was able to detect gammaDPGA in serum from infected animals at concentrations of 100 to 200 ng/mL. |
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Authors:
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David P AuCoin; Marjorie D Sutherland; Ann L Percival; C Rick Lyons; Julie A Lovchik; Thomas R Kozel |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2009-04-02 |
Journal Detail:
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Title: Diagnostic microbiology and infectious disease Volume: 64 ISSN: 1879-0070 ISO Abbreviation: Diagn. Microbiol. Infect. Dis. Publication Date: 2009 Jun |
Date Detail:
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Created Date: 2009-06-08 Completed Date: 2009-07-27 Revised Date: 2011-08-12 |
Medline Journal Info:
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Nlm Unique ID: 8305899 Medline TA: Diagn Microbiol Infect Dis Country: United States |
Other Details:
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Languages: eng Pagination: 229-32 Citation Subset: IM |
Affiliation:
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Department of Microbiology and Immunology, University of Nevada School of Medicine, Reno, 89557, USA. daucoin@medicine.nevada.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anthrax / diagnosis* Antigens, Bacterial / analysis*, immunology Bacillus anthracis / chemistry, immunology, isolation & purification* Bacterial Capsules / chemistry*, immunology Latex Fixation Tests / methods Mice Mice, Inbred BALB C Polyglutamic Acid / analogs & derivatives*, analysis, immunology Serum / chemistry |
| Grant Support | |
ID/Acronym/Agency:
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P20 RR-016464/RR/NCRR NIH HHS; R01 AI059348-04A2/AI/NIAID NIH HHS; R01 AI059348-06/AI/NIAID NIH HHS; R01 AI059348-07/AI/NIAID NIH HHS; R01-AI059348/AI/NIAID NIH HHS; U01 AI061200-05/AI/NIAID NIH HHS; U01-AI061200/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Bacterial; 0/Bacterial Capsules; 0/poly(gamma-glutamic acid); 25513-46-6/Polyglutamic Acid |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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