Document Detail


Rapid detection of food-borne pathogens by using molecular techniques.
MedLine Citation:
PMID:  15591255     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Traditional methods of identification of food-borne pathogens, which cause disease in humans, are time-consuming and laborious, so there is a need for the development of innovative methods for the rapid identification of food-borne pathogens. Recent advances in molecular cloning and recombinant DNA techniques have revolutionized the detection of pathogens in foods. In this study the development of a PCR-based technique for the rapid identification of the food-borne pathogens Salmonella and Escherichia coli was undertaken. Suitable primers were designed based on specific gene fimA of Salmonella and gene afa of pathogenic E. coli for amplification. Agarose gel electrophoresis and subsequent staining with ethidium bromide were used for the identification of PCR products. The size of the amplified product was 120 bp as shown by comparison with marker DNA. These studies have established that fimA and afa primers were specific for detecting Salmonella and pathogenic E. coli, respectively, in the environmental samples. Thus a rapid, sensitive and reliable technique for the detection of Salmonella and pathogenic E. coli was developed.
Authors:
Rambabu Naravaneni; Kaiser Jamil
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of medical microbiology     Volume:  54     ISSN:  0022-2615     ISO Abbreviation:  J. Med. Microbiol.     Publication Date:  2005 Jan 
Date Detail:
Created Date:  2004-12-13     Completed Date:  2005-02-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0224131     Medline TA:  J Med Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  51-4     Citation Subset:  IM    
Affiliation:
Department of Genetics, Bhagwan Mahavir Hospital and Research Centre, Masab tank, Hyderabad, Andhra Pradesh, India-500 004.
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MeSH Terms
Descriptor/Qualifier:
Adhesins, Escherichia coli / genetics
Antigens, Bacterial / genetics
DNA, Bacterial / analysis
Electrophoresis, Agar Gel
Escherichia coli / genetics,  isolation & purification*
Fimbriae Proteins / genetics
Fimbriae, Bacterial / genetics
Food Microbiology*
Polymerase Chain Reaction*
Salmonella / genetics,  isolation & purification*
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/Adhesins, Escherichia coli; 0/Antigens, Bacterial; 0/DNA, Bacterial; 0/SEF21 protein, Salmonella enteritidis; 147680-16-8/Fimbriae Proteins

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