Document Detail


Rapid detection of enterotoxigenic clostridium perfringens by real-time fluorescence resonance energy transfer PCR.
MedLine Citation:
PMID:  16786856     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Clostridium perfringens is one of the etiologic agents of gas gangrene that can occur when a wound is contaminated with soil. Type A C. perfringens can cause foodborne and nonfoodborne gastrointestinal illnesses due to an enterotoxin (CPE) produced by some strains during sporulation. We developed a quantitative real-time PCR assay based on fluorescence resonance energy transfer hybridization chemistry that targets the C. perfringens-specific phospholipase C (plc) gene and the enterotoxigenic gene (cpe) with the LightCycler and the Ruggedized Advanced Pathogen Identification Device (R.A.P.I.D.). The assay can detect as few as 20 copies of target sequences per PCR. The total assay time, from extraction to PCR analysis, is 90 min. This assay is rapid, sensitive, and specific and will allow direct detection of C. perfringens in water, food, and stool samples. It should prove helpful in investigating foodborne illnesses due to C. perfringens and can be used as a tool to ensure the safety of food and water supplies.
Authors:
Wilfred P dela Cruz; Mary M A Gozum; Sarah F Lineberry; Sarah D Stassen; Marianne Daughtry; Nicholas A Stassen; Morris S Jones; Oswald L Johnson
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of food protection     Volume:  69     ISSN:  0362-028X     ISO Abbreviation:  J. Food Prot.     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2006-06-21     Completed Date:  2006-07-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7703944     Medline TA:  J Food Prot     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1347-53     Citation Subset:  IM    
Affiliation:
Molecular Biology Research Element, Clinical Investigation Facility, David Grant USAF Medical Center, Travis Air Force Base, California 94535, USA. wilfred.delacruz@travis.af.mil
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Clostridium perfringens / classification,  isolation & purification*
DNA, Bacterial / analysis*
Enterotoxins / analysis,  genetics
Fluorescence
Food Contamination / analysis
Food Microbiology*
Molecular Sequence Data
Phylogeny
Polymerase Chain Reaction / methods*
Reproducibility of Results
Sensitivity and Specificity
Spores, Bacterial
Time Factors
Water Microbiology*
Chemical
Reg. No./Substance:
0/DNA, Bacterial; 0/Enterotoxins; 0/enterotoxin, Clostridium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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