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Rapid detection of ciprofloxacin effects on Fusarium graminearum and F. avenaceum cells in modulating environmental pH using a reactive, non-toxic food-dye indicator.
MedLine Citation:
PMID:  21843805     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The objective of the study was to assess the effect of ciprofloxacin antibiotic on the physiological or phenotypic characteristics of food-borne toxigenic Fusarium graminearum and F. avenaceum molds under in vitro conditions. In the presence of ciprofloxacin, Fusarium mycelia growth and morphology were altered based on the antibiotic concentration range used. Results showed that ciprofloxacin in concentrations ≥40μg/mL induced chlamydospore formation in Fusaria and as such, this antibiotic should be considered as an important abiotic stress factor and growth inhibitor. A novel method was investigated to correlate chlamydospore formation with the colour changes observed in FD&C Green Number 3, a common water soluble food dye. The antibiotic-treated F. graminearum and F. avenaceum isolates produced chamydospores, which in turn altered environmental pH with concomitant changes in the colour and intensity of the dye. The colour changes observed as a function of environmental pH were supported by instrumental methods (pH meter and spectroscopy), and a commercial pH indicator (thymol blue) results. In conclusion, we propose that FD&C Green Number 3 can be used as an accurate indicator for the rapid assessment of Fusarium molds when grown on ciprofloxacin antibiotic-containing substrate. Special emphasis should be given to an indirect risk assessment of antibiotic effects on toxic molds.
Authors:
Xiakun Yuan; Yit Kheng Goh; Nicholas Low; Vladimir Vujanovic
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Publication Detail:
Type:  Journal Article     Date:  2011-06-29
Journal Detail:
Title:  Environmental toxicology and pharmacology     Volume:  32     ISSN:  1872-7077     ISO Abbreviation:  Environ. Toxicol. Pharmacol.     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-08-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9612020     Medline TA:  Environ Toxicol Pharmacol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  242-8     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier B.V. All rights reserved.
Affiliation:
Department of Food & Bioproduct Sciences, University of Saskatchewan, 51 Campus Drive, S7N 5A8 Saskatoon, SK, Canada.
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