Document Detail

Rapid detection assays for multidrug resistance.
MedLine Citation:
PMID:  3245849     Owner:  NLM     Status:  MEDLINE    
In attempts to develop simple and rapid assays for detection of multidrug resistance (MDR) doxorubicin-resistant S180 (S180DOX), colchicine-resistant CHO (CHOCOL) and cytarabine (cytosine-arabinoside)-resistant L1210 cell lines (L1210AraC) were investigated. S180DOX and CHOCOL cells express the MDR-phenotype while L1210AraC does not. Using a previously described short-term assay firstly, inhibition of incorporation of radioactive nucleic acid precursors into tumour cells after addition of doxorubicin was measured. Secondly, the accumulation of the fluorescent dye rhodamine 123 (R123) in the different cell lines were analyzed. It could be observed that the resistant S180DOX and CHOCOL cells needed significantly more time to accumulate R123 than their sensitive parental cell lines or L1210AraC cells. Therefore, both assays are adequate tools for the rapid detection of MDR.
T Efferth; M Volm
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Arzneimittel-Forschung     Volume:  38     ISSN:  0004-4172     ISO Abbreviation:  Arzneimittelforschung     Publication Date:  1988 Dec 
Date Detail:
Created Date:  1989-06-02     Completed Date:  1989-06-02     Revised Date:  2006-04-24    
Medline Journal Info:
Nlm Unique ID:  0372660     Medline TA:  Arzneimittelforschung     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  1771-4     Citation Subset:  IM    
Institute of Experimental Pathology, German Cancer Research Center, Heidelberg.
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MeSH Terms
Antineoplastic Combined Chemotherapy Protocols / pharmacology*
Cell Line
Colchicine / pharmacology
Cytarabine / pharmacology
Dactinomycin / pharmacology
Doxorubicin / pharmacology
Drug Resistance
Leukemia L1210 / drug therapy
Rhodamine 123
Rhodamines / pharmacology
Sarcoma 180 / drug therapy
Vincristine / pharmacology
Reg. No./Substance:
0/Rhodamines; 147-94-4/Cytarabine; 23214-92-8/Doxorubicin; 50-76-0/Dactinomycin; 57-22-7/Vincristine; 62669-70-9/Rhodamine 123; 64-86-8/Colchicine

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