| Rapid detection assays for multidrug resistance. | |
| | |
MedLine Citation:
|
PMID: 3245849 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
In attempts to develop simple and rapid assays for detection of multidrug resistance (MDR) doxorubicin-resistant S180 (S180DOX), colchicine-resistant CHO (CHOCOL) and cytarabine (cytosine-arabinoside)-resistant L1210 cell lines (L1210AraC) were investigated. S180DOX and CHOCOL cells express the MDR-phenotype while L1210AraC does not. Using a previously described short-term assay firstly, inhibition of incorporation of radioactive nucleic acid precursors into tumour cells after addition of doxorubicin was measured. Secondly, the accumulation of the fluorescent dye rhodamine 123 (R123) in the different cell lines were analyzed. It could be observed that the resistant S180DOX and CHOCOL cells needed significantly more time to accumulate R123 than their sensitive parental cell lines or L1210AraC cells. Therefore, both assays are adequate tools for the rapid detection of MDR. |
| | |
Authors:
|
T Efferth; M Volm |
Related Documents
:
|
9788569 - Lack of correlation of mrp and gamma-glutamylcysteine synthetase overexpression with do... 8337699 - Modification of mipafox-induced inhibition of neuropathy target esterase in neuroblasto... 14641819 - Modulation of resistance to idarubicin by the cyclosporin psc 833 (valspodar) in multid... 12439919 - Mechanism of 5-fluorouracil required resistance in human hepatocellular carcinoma cell ... 11314019 - Inhibition of nf-kappab sensitizes human pancreatic carcinoma cells to apoptosis induce... 2005419 - Efflux of potassium ions in angiotensin ii-stimulated bovine adrenocortical cells. 16280349 - Mis-specified cells die by an active gene-directed process, and inhibition of this deat... 22790669 - Exposure of primary porcine urothelial cells to benzo(a)pyrene: in vitro uptake, intrac... 2072409 - Different metastasis patterns of a human melanoma cell line in nude mice and rats: infl... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Arzneimittel-Forschung Volume: 38 ISSN: 0004-4172 ISO Abbreviation: Arzneimittelforschung Publication Date: 1988 Dec |
Date Detail:
|
Created Date: 1989-06-02 Completed Date: 1989-06-02 Revised Date: 2006-04-24 |
Medline Journal Info:
|
Nlm Unique ID: 0372660 Medline TA: Arzneimittelforschung Country: GERMANY, WEST |
Other Details:
|
Languages: eng Pagination: 1771-4 Citation Subset: IM |
Affiliation:
|
Institute of Experimental Pathology, German Cancer Research Center, Heidelberg. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Antineoplastic Combined Chemotherapy Protocols / pharmacology* Cell Line Colchicine / pharmacology Cricetinae Cricetulus Cytarabine / pharmacology Dactinomycin / pharmacology Doxorubicin / pharmacology Drug Resistance Female Leukemia L1210 / drug therapy Rhodamine 123 Rhodamines / pharmacology Sarcoma 180 / drug therapy Vincristine / pharmacology |
| Chemical | |
Reg. No./Substance:
|
0/Rhodamines; 147-94-4/Cytarabine; 23214-92-8/Doxorubicin; 50-76-0/Dactinomycin; 57-22-7/Vincristine; 62669-70-9/Rhodamine 123; 64-86-8/Colchicine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Internally standardized method for the determination of nalbuphine in human plasma by means of high ...
Next Document: Use of sorption promoters to increase systemic absorption of coumarin from transdermal drug delivery...