Document Detail


Rapid detection of Cronobacter spp. with a method combining impedance technology and rRNA based lateral flow assay.
MedLine Citation:
PMID:  22917579     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Cronobacter spp. is an important release test parameter for powered infant formula (PIF). An impedance method is proposed for the rapid detection of this pathogen in PIF. An impedance based method (BacTrac 4300 Microbiological Analyzer) combined with a RNA hybridisation assay (RiboFlow™) was evaluated using 23 strains in PIF samples and compared to a culture based reference method (ISO/TS 22964). The influences of competitive flora, heat and dry stress on the reliability of the impedance method were investigated. Seven different Cronobacter species were included in the evaluation, among them are strains with high susceptibility to low pH and high temperatures. Compared to the reference method, a higher sensitivity (85%) and specificity (100%) was observed using the impedance method, combined with the commercial rRNA based lateral flow test kit as a confirmation tool. The detection time was substantially shortened by using the impedance technique and RiboFlow™. Cronobacter could be detected within maximally 29h, while the reference method takes up to five days when including confirmation of positive results.
Authors:
Sha Zhu; Sylvia Schnell; Matthias Fischer
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-28
Journal Detail:
Title:  International journal of food microbiology     Volume:  -     ISSN:  1879-3460     ISO Abbreviation:  Int. J. Food Microbiol.     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-8-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8412849     Medline TA:  Int J Food Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier B.V.
Affiliation:
Central Laboratories Friedrichsdorf, Bahnstr. 14-30, 61381 Friedrichsdorf, Germany; Institute for Applied Microbiology, Justus-Liebig University Giessen, Heinrich-Buff-Ring 26-32 (IFZ), D-35392 Giessen, Germany.
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