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Rapid de-esterification and loss of eicosapentaenoic acid from rat brain phospholipids: an intracerebroventricular study.
MedLine Citation:
PMID:  21091476     Owner:  NLM     Status:  In-Process    
Eicosapentaenoic acid (EPA, 20:5n-3) is being explored as a therapy in neurological diseases and disorders. Although it is known that palmitate is the most abundant fatty acid in the brain while EPA is one of the lowest, the mechanism by which the brain maintains this balance is unclear. Therefore, to trace the metabolism of these fatty acids in the brain, (14) C-palmitate or (14) C-EPA was administered via intracerebroventricular infusion to rats. From 4 to 128 days post-infusion, brains were collected after head-focused, high-energy microwave irradiation for biochemical analysis. At day 4 post-infusion, 57% (82 ± 26 nCi) of the total phospholipid radioactivity in (14) C-palmitate-infused brains was intact palmitate; whereas in (14) C-EPA-infused brains, 9% (2 ± 0.9 nCi) of the radioactivity was intact EPA. The half-life of esterified (14) C-palmitate and (14) C-EPA was 32 ± 4 (2% loss per day) and 5 ± 0.2 days (14% loss per day), respectively. Radioactivity was also detected in other saturates, monounsaturates, and cholesterol, suggesting that the infused radiolabeled fatty acids were β-oxidized. In conclusion, the low concentration of EPA in brain phospholipids may be the result of extensive metabolism of EPA, in part by β-oxidation, upon entry into the brain and upon de-esterification from phospholipids.
Chuck T Chen; Zhen Liu; Richard P Bazinet
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-12-22
Journal Detail:
Title:  Journal of neurochemistry     Volume:  116     ISSN:  1471-4159     ISO Abbreviation:  J. Neurochem.     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-01-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985190R     Medline TA:  J Neurochem     Country:  England    
Other Details:
Languages:  eng     Pagination:  363-73     Citation Subset:  IM    
Copyright Information:
© 2010 The Authors. Journal of Neurochemistry © 2010 International Society for Neurochemistry.
Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, Toronto, ON, Canada.
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