Document Detail

Rapid and convenient method for preparing aurapten-enriched product from hassaku peel oil: implications for cancer-preventive food additives.
MedLine Citation:
PMID:  12009985     Owner:  NLM     Status:  MEDLINE    
Aurapten (7-geranyloxycoumarin) has been reported to be an effective inhibitor of chemical carcinogenesis in some rodent models. In the present study, a method for preparing an aurapten-enriched agricultural product has been established. Out of 17 Rutaceae varieties, the aurapten content in hassaku (Citrus hassaku Hort ex Y. Tanaka) fruit peel was marked, as well as that in natsumikan (C. natsudaidai) and grapefruit (C. paradisi). The aurapten content in hassaku peel was most abundant in April. Hassaku fruit peel oil, which was dissolved by heating precipitates including aurapten which had formed after freezing the peel oil at -20 degrees C, was used. After adsorbing aurapten from peel oil onto synthetic adsorbent SP70, the adsorbent was washed with 40% (v/v) ethanol in water to remove essential oils and pigments remaining on the adsorbent. Aurapten was then eluted with 80% (v/v) ethanol. In a laboratory-scale test, the recovery rates of aurapten and total carotenoids from the eluates were 74.3 and 4.6%, respectively. In a pilot-scale test, the recovery rate of aurapten in the aurapten-enriched preparation from dissolved hassaku oil was 91.0%, and its concentration was 64.1% (w/w). When stored for 180 days under sunlight, aurapten in powder form remained at 88.0-89.0% of the initial level, but only 31.3-43.8% in ethanol. The stability of aurapten in the aurapten-enriched preparation was higher than that of purified aurapten. These results suggest that aurapten is readily recovered from hassaku peel oil using SP70, and thus may be used as a food additive.
Yasuo Takahashi; Nobuya Inaba; Shigeru Kuwahara; Wataru Kuki; Keiko Yamane; Akira Murakami
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  50     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2002 May 
Date Detail:
Created Date:  2002-05-15     Completed Date:  2002-07-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3193-6     Citation Subset:  IM    
Wakayama Agricultural Processing Research Corporation, 398 Tsukatsuki, Momoyama-Chou, Naga-Gun, Wakayama 649-61, Japan.
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MeSH Terms
Anticarcinogenic Agents / analysis*
Chromatography, High Pressure Liquid
Citrus / chemistry*
Coumarins / analysis*
Drug Stability
Food Additives*
Fruit / chemistry*
Plant Oils / analysis*
Reg. No./Substance:
0/Anticarcinogenic Agents; 0/Coumarins; 0/Food Additives; 0/Plant Oils; 495-02-3/aurapten

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