Document Detail

Rapid and complete degradation of thymidine by human peripheral blood platelets: implications for genotoxicity assays.
MedLine Citation:
PMID:  3748055     Owner:  NLM     Status:  MEDLINE    
Small-scale washed cell preparations obtained by Percoll density-gradient fractionation of whole blood were used to study the metabolic fate of [3H]thymidine supplied to isolated human blood mononuclear cells and platelets incubated for up to 24 h in vitro. Two cell fractions were monitored: low molecular weight compounds which were soluble in Triton X-100 and TCA were investigated by thin-layer chromatography, and high molecular weight components, distinguished by their Triton and TCA insolubility, were examined by agarose-gel electrophoresis. Under the conditions used, greater than 99% of added [3H]thymidine was very rapidly degraded. Catabolites were recovered in the Triton-soluble (cytoplasmic) fraction and the extracellular medium. A negligible proportion of added label was associated with Triton- and TCA-insoluble cell fractions. These results confirm and clarify previous data and have important implications for genotoxicity tests which employ in vitro leukocyte cultures.
T Shaw; D G MacPhee
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Mutation research     Volume:  163     ISSN:  0027-5107     ISO Abbreviation:  Mutat. Res.     Publication Date:  1986 Oct 
Date Detail:
Created Date:  1986-10-22     Completed Date:  1986-10-22     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0400763     Medline TA:  Mutat Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  75-80     Citation Subset:  IM    
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MeSH Terms
Blood Platelets / metabolism*
DNA Repair
Leukocytes / metabolism
Mutagenicity Tests / standards*
Thymidine / metabolism*
Thymine / metabolism
Reg. No./Substance:
50-89-5/Thymidine; 65-71-4/Thymine

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