| Rapid cold-hardening protects Drosophila melanogaster from cold-induced apoptosis. | |
| | |
MedLine Citation:
|
PMID: 17245639 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The rapid cold-hardening (RCH) response increases the cold tolerance of insects by protecting against non-freezing, cold-shock injury. Apoptosis, or programmed cell death, plays important roles in development and the elimination of sub-lethally damaged cells. Our objectives were to determine whether apoptosis plays a role in cold-shock injury and, if so, whether the RCH response protects against cold-induced apoptosis in Drosophila melanogaster. The present study confirmed that RCH increased the cold tolerance of the adults at the organismal level. No flies in the cold-shocked group survived direct exposure to 7 degrees C for 2 h, whereas significantly more flies in the RCH group survived exposure to 7 degrees C for 2 h after a 2-h exposure to 5 degrees C. We used a TUNEL assay to detect and quantify apoptotic cell death in five groups of flies including control, cold-shocked, RCH, heat-shocked (37.5 degrees C, 30 min), and frozen (20 degrees C, 24 h) and found that apoptosis was induced by cold shock, heat shock, and freezing. The RCH treatment significantly improved cell viability by 38% compared to the cold-shocked group. Cold shock-induced DNA fragmentation shown by electrophoresis provided further evidence for apoptosis. SDS-PAGE analysis revealed an RCH-specific protein band with molecular mass of approximately 150 kDa. Western-blotting revealed three proteins that play key roles in the apoptotic pathway: caspase-9-like (apoptotic initiator), caspase-3-like (apoptotic executioner) and Bcl-2 (anti-apoptotic protein). Consequently, the results of this study support the hypothesis that the RCH response protects against cold-shock-induced apoptosis. |
| | |
Authors:
|
Shu-Xia Yi; Clifford W Moore; Richard E Lee |
Related Documents
:
|
9949169 - Enhanced endothelial cell apoptosis in splenic tissues of patients with thrombotic thro... 15325289 - Isolation and characterization of mammalian cells that are undergoing apoptosis by a bo... 15659799 - Enhanced expression of natural resistance-associated macrophage protein 1 in atheroscle... 12084619 - Amiodarone induces apoptosis in l-132 human lung epithelial cell line. 9450519 - Annexin v-affinity assay: a review on an apoptosis detection system based on phosphatid... 18958689 - Use of avian lymphocytes to detect toxicity: effects of a commonly utilized deltamethri... 19190769 - Physiological role of plasmacytoid dendritic cells and their potential use in cancer im... 22215029 - Visualization of vascular ca2+ signaling triggered by paracrine derived ros. 14731549 - A role for oxygen radicals as second messengers. |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
|
Title: Apoptosis : an international journal on programmed cell death Volume: 12 ISSN: 1360-8185 ISO Abbreviation: Apoptosis Publication Date: 2007 Jul |
Date Detail:
|
Created Date: 2007-06-11 Completed Date: 2007-11-13 Revised Date: 2008-11-21 |
Medline Journal Info:
|
Nlm Unique ID: 9712129 Medline TA: Apoptosis Country: United States |
Other Details:
|
Languages: eng Pagination: 1183-93 Citation Subset: IM |
Affiliation:
|
Department of Zoology, Miami University, Oxford, OH, 45056, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Apoptosis / physiology* Caspase 3 / metabolism Caspase 9 / metabolism Cold Temperature* Drosophila melanogaster / cytology, physiology* Heat-Shock Proteins / metabolism In Situ Nick-End Labeling Inhibitor of Apoptosis Proteins / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Heat-Shock Proteins; 0/Inhibitor of Apoptosis Proteins; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 9 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Owner attitudes and self reported behavior towards modified work after occupational injury absence i...
Next Document: The apoptotic microtubule network preserves plasma membrane integrity during the execution phase of ...