Document Detail

Rapid cold-hardening of Drosophila melanogaster (Diptera: Drosophiladae) during ecologically based thermoperiodic cycles.
MedLine Citation:
PMID:  11398755     Owner:  NLM     Status:  MEDLINE    
In contrast to most studies of rapid cold-hardening, in which abrupt transfers to low temperatures are used to induce an acclimatory response, the primary objectives of this study were to determine (i) whether rapid cold-hardening was induced during the cooling phase of an ecologically based thermoperiod, (ii) whether the protection afforded was lost during warming or contributed to increased cold-tolerance during subsequent cycles and (iii) whether the major thermally inducible stress protein (Hsp70) or carbohydrate cryoprotectants contributed to the protection afforded by rapid cold-hardening. During the cooling phase of a single ecologically based thermoperiod, the tolerance of Drosophila melanogaster to 1 h at -7 degrees C increased from 5 +/- 5% survival to 62.5 +/- 7.3% (means +/- S.E.M., N=40-60), while their critical thermal minima (CTmin) decreased by 1.9 degrees C. Cold hardiness increased with the number of thermoperiods to which flies were exposed; i.e. flies exposed to six thermoperiods were more cold-tolerant than those exposed to two. Endogenous levels of Hsp70 and carbohydrate cryoprotectants were unchanged in rapidly cold-hardened adults compared with controls held at a constant 23 degrees C. In nature, rapid cold-hardening probably affords subtle benefits during short-term cooling, such as allowing D. melanogaster to remain active at lower temperatures than they otherwise could.
J D Kelty; R E Lee
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  The Journal of experimental biology     Volume:  204     ISSN:  0022-0949     ISO Abbreviation:  J. Exp. Biol.     Publication Date:  2001 May 
Date Detail:
Created Date:  2001-06-08     Completed Date:  2001-07-19     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0243705     Medline TA:  J Exp Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1659-66     Citation Subset:  IM    
Department of Zoology, 212 Pearson Hall, Miami University, Oxford, OH 45056, USA.
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MeSH Terms
Carbohydrate Metabolism
Cold Temperature*
Cryoprotective Agents / metabolism
Drosophila melanogaster / physiology*
HSP70 Heat-Shock Proteins / metabolism
Hot Temperature
Sex Characteristics
Reg. No./Substance:
0/Cryoprotective Agents; 0/HSP70 Heat-Shock Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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