Document Detail


Rapid changes in anterior pituitary cell phenotypes in male and female mice after acute cold stress.
MedLine Citation:
PMID:  18202140     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The anterior pituitary (AP) is made of five different cell types. The relative abundance and phenotype of AP cells may change in different physiological situations as an expression of pituitary plasticity. Here, we analyze in detail the phenotype of mouse corticotropes and the effects of acute cold stress on AP cell populations. The hormone content and the expression of hypothalamic-releasing hormone (HRH) receptors in all the five AP cell types were studied in the male and female mice at rest and after a 30-min cold stress. Expression of HRH receptors was evidenced by imaging the single-cell cytosolic Ca(2+) responses in fura-2-loaded cells. Hormone contents were studied by multiple, simultaneous immunofluorescence of all the five hormones. Corticotropes displayed a striking sexual dimorphism, even in the resting condition. Male corticotropes showed the orthodox phenotype. They were monohormonal, storing only ACTH, and monoreceptorial, responding only to CRH. In contrast, female corticotropes were made of about equal parts of orthodox cells and multifunctional cells, which co-stored additional AP hormones and expressed additional HRH receptors. Cold stress did not modify the number of ACTH containing cells, but, according to immunostaining, it increased the relative abundance of other AP cell types at the expense of the pool of cells storing no hormones. Cold stress also modified the response to CRH and other HRHs. Most of these phenotypical changes presented a strong sexual dimorphism. These results indicate that pituitary plasticity is even larger than previously thought.
Authors:
Laura Senovilla; Lucía Núñez; Carlos Villalobos; Javier García-Sancho
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-01-17
Journal Detail:
Title:  Endocrinology     Volume:  149     ISSN:  0013-7227     ISO Abbreviation:  Endocrinology     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-04-22     Completed Date:  2008-06-10     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2159-67     Citation Subset:  AIM; IM    
Affiliation:
Instituto de Biología y Genética Molecular, Universidad de Valladolid, C/ Sanz y Forés s/n, 47003 Valladolid, Spain.
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MeSH Terms
Descriptor/Qualifier:
Adaptation, Physiological / physiology
Adrenocorticotropic Hormone / metabolism
Animals
Cells, Cultured
Cold Temperature*
Corticotropin-Releasing Hormone / metabolism
Female
Gonadotropin-Releasing Hormone / metabolism
Growth Hormone-Releasing Hormone / metabolism
Luteinizing Hormone / metabolism
Male
Mice
Phenotype
Pituitary Gland, Anterior / cytology,  metabolism,  physiology*
Prolactin / metabolism
Sex Characteristics
Stress, Physiological / metabolism,  physiopathology*
Thyrotropin-Releasing Hormone / metabolism
Time Factors
Chemical
Reg. No./Substance:
24305-27-9/Thyrotropin-Releasing Hormone; 33515-09-2/Gonadotropin-Releasing Hormone; 9002-60-2/Adrenocorticotropic Hormone; 9002-62-4/Prolactin; 9002-67-9/Luteinizing Hormone; 9015-71-8/Corticotropin-Releasing Hormone; 9034-39-3/Growth Hormone-Releasing Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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