| Rapid blood separation is superior to fluoride for preventing in vitro reductions in measured blood glucose concentration. | |
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MedLine Citation:
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PMID: 19638548 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIMS: To determine whether tubes containing sodium fluoride negatively bias blood glucose concentration by directly comparing glucose concentrations in paired blood samples collected in tubes containing lithium heparin (Li-Heparin) and tubes containing sodium fluoride/potassium oxalate (NaF-KOx). METHODS: Paired blood samples from a group of patients (n = 1040) were collected in tubes containing Li-Heparin and tubes containing NaF-KOx at the same time. All Li-Heparin samples were centrifuged soon after collection and were kept cool in transport along with NaF-KOx samples, which were centrifuged at the receiving location after an average transport time of 4 h, but immediately before analysis. Glucose concentrations in the paired samples were determined simultaneously by an automated oxidase method. RESULTS: The mean glucose concentrations for NaF-KOx samples and Li-Heparin samples were 5.7 mmol/l and 6.1 mmol/l, respectively, with a mean difference of 0.39 mmol/l. CONCLUSION: Rapid separation of heparinised blood is superior to fluoride alone for abrogating glycolytic effects on blood glucose measurements in the clinical laboratory. |
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Authors:
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R Z Shi; E S Seeley; R Bowen; J D Faix |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Journal of clinical pathology Volume: 62 ISSN: 1472-4146 ISO Abbreviation: J. Clin. Pathol. Publication Date: 2009 Aug |
Date Detail:
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Created Date: 2009-07-29 Completed Date: 2009-11-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0376601 Medline TA: J Clin Pathol Country: England |
Other Details:
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Languages: eng Pagination: 752-3 Citation Subset: AIM; IM |
Affiliation:
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Department of Pathology, Stanford University, Stanford, California, USA. rzshi@stanford.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Blood Glucose
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analysis*,
drug effects Blood Specimen Collection / methods* Centrifugation Glycolysis / drug effects Heparin / pharmacology Humans Lithium / pharmacology Reproducibility of Results Sodium Fluoride / pharmacology* Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Blood Glucose; 7439-93-2/Lithium; 7681-49-4/Sodium Fluoride; 9005-49-6/Heparin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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