Document Detail


Rapid biochemical screening for Salmonella, Shigella, Yersinia, and Aeromonas isolates from stool specimens.
MedLine Citation:
PMID:  8077408     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Four screens for the rapid (4 to 6 h) biochemical detection of pathogens from enteric isolation media are described. The Salmonella screen consisted of Kligler iron agar (KIA), motility-indole-urea-tryptophan-deamination semisolid medium (MIU-TDA), and the o-nitrophenyl-beta-D-galactopyranoside (ONPG) test; the Shigella screen consisted of KIA, MIU-TDA, the ONPG test, and the lysine decarboxylation-indole test; the Yersinia screen consisted of a rhamnose broth; the Aeromonas screen consisted of a xylose agar plate. When tested on 2,102 fresh isolates and 71 stock strains, the screens correctly detected 212 enteric pathogens (sensitivity, 100%), with a specificity of 98.1%.
Authors:
R De Ryck; M J Struelens; E Serruys
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  32     ISSN:  0095-1137     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  1994 Jun 
Date Detail:
Created Date:  1994-10-06     Completed Date:  1994-10-06     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1583-5     Citation Subset:  IM    
Affiliation:
Laboratoire de Microbiologie, Hôpital Erasme, Université Libre de Bruxelles, Belgium.
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MeSH Terms
Descriptor/Qualifier:
Aeromonas / isolation & purification*
Algorithms
Culture Media
Dysentery, Bacillary / diagnosis*,  microbiology
Evaluation Studies as Topic
Feces / microbiology*
Gram-Negative Bacterial Infections / diagnosis*,  microbiology
Humans
Salmonella / isolation & purification*
Salmonella Infections / diagnosis*,  microbiology
Sensitivity and Specificity
Shigella / isolation & purification*
Time Factors
Yersinia / isolation & purification*
Yersinia Infections / diagnosis*,  microbiology
Chemical
Reg. No./Substance:
0/Culture Media
Comments/Corrections

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