| Rapid assay for pathogenic Salmonella organisms by immunofluorescence flow cytometry. | |
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MedLine Citation:
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PMID: 7799425 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Multi-parameter flow cytometry was investigated for the rapid detection of specific serotypes of salmonellas (S. typhimurium and S. montevideo) labelled with fluorescent monoclonal antibodies, both in pure culture and in a typical food matrix (full-fat milk). In all cases, the method was accurate to levels of below 10(4) target cells per ml for a total assay time of about 30 min. After 6 h non-selective enrichment in the presence of a 10,000-fold excess of competing micro-organisms (Escherichia coli) the corresponding detection limit was about 20 cells ml-1. These results suggest that flow cytometry has significant potential for the detection of pathogenic micro-organisms in the food industry. |
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Authors:
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A C Pinder; R G McClelland |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of microscopy Volume: 176 ISSN: 0022-2720 ISO Abbreviation: J Microsc Publication Date: 1994 Oct |
Date Detail:
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Created Date: 1995-01-26 Completed Date: 1995-01-26 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0204522 Medline TA: J Microsc Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 17-22 Citation Subset: IM |
Affiliation:
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Department of Food Biophysics, Norwich Laboratory, U.K. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Bacterial Antibodies, Monoclonal Colony Count, Microbial Escherichia coli / growth & development, immunology, isolation & purification Flow Cytometry / methods* Fluorescent Antibody Technique* Food Microbiology* Milk / microbiology Salmonella / growth & development, immunology, isolation & purification* Salmonella typhimurium / growth & development, immunology, isolation & purification |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Bacterial; 0/Antibodies, Monoclonal |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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