Document Detail


Rapid assay for pathogenic Salmonella organisms by immunofluorescence flow cytometry.
MedLine Citation:
PMID:  7799425     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Multi-parameter flow cytometry was investigated for the rapid detection of specific serotypes of salmonellas (S. typhimurium and S. montevideo) labelled with fluorescent monoclonal antibodies, both in pure culture and in a typical food matrix (full-fat milk). In all cases, the method was accurate to levels of below 10(4) target cells per ml for a total assay time of about 30 min. After 6 h non-selective enrichment in the presence of a 10,000-fold excess of competing micro-organisms (Escherichia coli) the corresponding detection limit was about 20 cells ml-1. These results suggest that flow cytometry has significant potential for the detection of pathogenic micro-organisms in the food industry.
Authors:
A C Pinder; R G McClelland
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of microscopy     Volume:  176     ISSN:  0022-2720     ISO Abbreviation:  J Microsc     Publication Date:  1994 Oct 
Date Detail:
Created Date:  1995-01-26     Completed Date:  1995-01-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0204522     Medline TA:  J Microsc     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  17-22     Citation Subset:  IM    
Affiliation:
Department of Food Biophysics, Norwich Laboratory, U.K.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Bacterial
Antibodies, Monoclonal
Colony Count, Microbial
Escherichia coli / growth & development,  immunology,  isolation & purification
Flow Cytometry / methods*
Fluorescent Antibody Technique*
Food Microbiology*
Milk / microbiology
Salmonella / growth & development,  immunology,  isolation & purification*
Salmonella typhimurium / growth & development,  immunology,  isolation & purification
Chemical
Reg. No./Substance:
0/Antibodies, Bacterial; 0/Antibodies, Monoclonal

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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