| Rapid analysis of whole blood by paper spray mass spectrometry for point-of-care therapeutic drug monitoring. | |
| | |
MedLine Citation:
|
PMID: 22479698 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Paper spray mass spectrometry is applied to oncology drugs in fresh whole blood samples supported on filter paper substrates instead of dry blood as done previously. Addition of the coagulant alum clotted the blood and allowed for immediate sample analysis. The coagulant did not interfere with the function of the paper spray nor did it add features to the mass spectra. Quantitative analysis of therapeutic drugs in the blood was achieved utilizing internal standards which were pre-spotted onto the filter paper. Eight oncology drugs were examined, with lower limits of detection ranging between 0.5 and 17 ng mL(-1) and linear dynamic ranges greater than two orders of magnitude. Inter-day accuracies of quality controls for pazopanib ranged from 102 to 118%, with imprecisions of 9 to 13%. This one-step method requires 10 μL of blood, a drop of solvent, and takes 45 seconds per trial. These results indicate applicability to point-of-care therapeutic drug monitoring in a clinical setting. |
| | |
Authors:
|
Ryan D Espy; Nicholas E Manicke; Zheng Ouyang; R Graham Cooks |
Related Documents
:
|
14965638 - Amlodipine gynaecomastia. 22842708 - Biological network modeling identifies ipcs in leishmania as a therapeutic target. 22594988 - Mthpc - a drug on its way from second to third generation photosensitizer? 22673828 - Current perspectives on herb-drug interactions in the european regulatory landscape. 8831728 - Effects of membrane potential versus phi on the cellular retention of doxorubicin analy... 2692318 - Dose-response relationship in the treatment of gastrointestinal disorders. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-4-5 |
Journal Detail:
|
Title: The Analyst Volume: - ISSN: 1364-5528 ISO Abbreviation: - Publication Date: 2012 Apr |
Date Detail:
|
Created Date: 2012-4-5 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0372652 Medline TA: Analyst Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA. cooks@purdue.edu. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Molecularly imprinted solid-phase extraction and high-performance liquid chromatography with ultravi...
Next Document: Fluorescence excitation-emission matrix (EEM) spectroscopy and cavity ring-down (CRD) absorption spe...