Document Detail

Rapid analysis of fatty acids in plasma lipids.
MedLine Citation:
PMID:  2280679     Owner:  NLM     Status:  MEDLINE    
A rapid and convenient procedure for the quantitative determination of the fatty acid composition of plasma lipids is described. Human plasma was applied directly to the preadsorbent zones of thin-layer silica gel plates with added antioxidant, internal standards and carriers. The thin-layer chromatography (TLC) plates were partially developed with methanol followed by chloroform/methanol (1:1, v/v), and then they were fully developed in hexane/diethyl ether/acetic acid (80:20:1, v/v/v) to separate the major classes of lipids. Silica gel from regions containing the separated lipids was scraped into screw-capped tubes and treated with boron trifluoride-methanol prior to gas chromatography. The method of direct application to TLC plates gave yields and compositions of fatty acids very similar to the method of applying extracted plasma lipids. This relatively simple method is suitable for analyzing the fatty acids in plasma lipids from a 50 microliter finger-tip blood samples from an individual, and it may be useful in wide-scale screening of different individuals to estimate the relative amounts of ingested polyunsaturated fatty acids.
A Ohta; M C Mayo; N Kramer; W E Lands
Related Documents :
21282499 - Omega-3 fatty acids prevent pressure overload-induced cardiac fibrosis through activati...
3958609 - Direct transesterification of all classes of lipids in a one-step reaction.
10349739 - Comparative immunological and chemical analysis of lipids and carotenoids of the d1-pep...
3429239 - Metabolism of beta-methyl[1-11c]heptadecanoic acid in canine myocardium.
7605209 - Fatty acid profiles of invertebrate iridescent viruses.
7171289 - Lipids in the classification of nocardioides: reclassification of arthrobacter simplex ...
20547249 - Structural basis for conservation in the cyp51 family.
18961019 - Microdetermination of alpha-amino acids by spectrophotometric and titrimetric methods.
12898349 - Decreased lysophosphatidylcholine/phosphatidylcholine ratio in cerebrospinal fluid in a...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Lipids     Volume:  25     ISSN:  0024-4201     ISO Abbreviation:  Lipids     Publication Date:  1990 Nov 
Date Detail:
Created Date:  1991-03-12     Completed Date:  1991-03-12     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0060450     Medline TA:  Lipids     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  742-7     Citation Subset:  IM    
Department of Biological Chemistry, University of Illinois, Chicago 60680.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Chromatography, Gas
Chromatography, Thin Layer
Fatty Acids / blood*
Lipids / blood,  chemistry*
Time Factors
Grant Support
Reg. No./Substance:
0/Fatty Acids; 0/Lipids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Analysis of cholesterol and desmosterol in cultured cells without organic solvent extraction.
Next Document:  A method for the quantitative analysis of molecular species of alkylacylglycerol and diacylglycerol.