Document Detail


Rapid analysis of fatty acids in plasma lipids.
MedLine Citation:
PMID:  2280679     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A rapid and convenient procedure for the quantitative determination of the fatty acid composition of plasma lipids is described. Human plasma was applied directly to the preadsorbent zones of thin-layer silica gel plates with added antioxidant, internal standards and carriers. The thin-layer chromatography (TLC) plates were partially developed with methanol followed by chloroform/methanol (1:1, v/v), and then they were fully developed in hexane/diethyl ether/acetic acid (80:20:1, v/v/v) to separate the major classes of lipids. Silica gel from regions containing the separated lipids was scraped into screw-capped tubes and treated with boron trifluoride-methanol prior to gas chromatography. The method of direct application to TLC plates gave yields and compositions of fatty acids very similar to the method of applying extracted plasma lipids. This relatively simple method is suitable for analyzing the fatty acids in plasma lipids from a 50 microliter finger-tip blood samples from an individual, and it may be useful in wide-scale screening of different individuals to estimate the relative amounts of ingested polyunsaturated fatty acids.
Authors:
A Ohta; M C Mayo; N Kramer; W E Lands
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Lipids     Volume:  25     ISSN:  0024-4201     ISO Abbreviation:  Lipids     Publication Date:  1990 Nov 
Date Detail:
Created Date:  1991-03-12     Completed Date:  1991-03-12     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0060450     Medline TA:  Lipids     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  742-7     Citation Subset:  IM    
Affiliation:
Department of Biological Chemistry, University of Illinois, Chicago 60680.
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MeSH Terms
Descriptor/Qualifier:
Chromatography, Gas
Chromatography, Thin Layer
Fatty Acids / blood*
Humans
Lipids / blood,  chemistry*
Methylation
Time Factors
Grant Support
ID/Acronym/Agency:
HL-34045/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Lipids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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