| Rapid analysis of cholesterol-elevating compounds in coffee brews by off-line high-performance liquid chromatography/high-resolution gas chromatography. | |
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MedLine Citation:
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PMID: 10564010 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A new method is proposed to analyze the cholesterol-elevating cafestol and kahweol which allows their rapid and reliable determination in different coffee brews. The method involves the preseparation of the sample by high-performance liquid chromatography, the collection of the selected fraction, and its subsequent analysis by high-resolution gas chromatography using a programmed temperature vaporizer operated in the split mode as sampling system. Under the experimental conditions investigated, recoveries as high as 87% (cafestol) and 94% (kahweol) were achieved while detection limits equal to 0.06 and 0.04 ppm for cafestol and kahweol, respectively, were obtained. Examples are given comparing levels of cafestol and kahweol resulting from the same ground roasted coffee by different brewing methods, which show the lowest values for brews prepared from coffee bags. |
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Authors:
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M L Ruiz del Castillo; M Herraiz; G P Blanch |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of agricultural and food chemistry Volume: 47 ISSN: 0021-8561 ISO Abbreviation: J. Agric. Food Chem. Publication Date: 1999 Apr |
Date Detail:
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Created Date: 2000-08-22 Completed Date: 2000-08-22 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1525-9 Citation Subset: IM |
Affiliation:
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Instituto de Fermentaciones Industriales, Madrid, Spain. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cholesterol
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blood Chromatography, Gas / methods Chromatography, High Pressure Liquid / methods Coffee / chemistry* Cookery Diterpenes / analysis* Food Handling Humans |
| Chemical | |
Reg. No./Substance:
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0/Coffee; 0/Diterpenes; 469-83-0/cafestol; 57-88-5/Cholesterol; 6894-43-5/kahweol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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