|Rapid adaptation to food availability by a dopamine-mediated morphogenetic response.|
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|PMID: 22186888 Owner: NLM Status: MEDLINE|
|Food can act as a powerful stimulus, eliciting metabolic, behavioural and developmental responses. These phenotypic changes can alter ecological and evolutionary processes; yet, the molecular mechanisms underlying many plastic phenotypic responses remain unknown. Here we show that dopamine signalling through a type-D(2) receptor mediates developmental plasticity by regulating arm length in pre-feeding sea urchin larvae in response to food availability. Although prey-induced traits are often thought to improve food acquisition, the mechanism underlying this plastic response acts to reduce feeding structure size and subsequent feeding rate. Consequently, the developmental programme and/or maternal provisioning predetermine the maximum possible feeding rate, and food-induced dopamine signalling reduces food acquisition potential during periods of abundant resources to preserve maternal energetic reserves. Sea urchin larvae may have co-opted the widespread use of food-induced dopamine signalling from behavioural responses to instead alter their development.|
|Diane K Adams; Mary A Sewell; Robert C Angerer; Lynne M Angerer|
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|Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2011-12-20|
|Title: Nature communications Volume: 2 ISSN: 2041-1723 ISO Abbreviation: Nat Commun Publication Date: 2011|
|Created Date: 2011-12-21 Completed Date: 2012-04-02 Revised Date: 2014-04-21|
Medline Journal Info:
|Nlm Unique ID: 101528555 Medline TA: Nat Commun Country: England|
|Languages: eng Pagination: 592 Citation Subset: IM|
|APA/MLA Format Download EndNote Download BibTex|
Dopamine / metabolism*
Dopamine Agonists / pharmacology
Dopamine Antagonists / pharmacology
Larva / anatomy & histology*, physiology
Morphogenesis / physiology*
Receptors, Dopamine D2 / agonists, antagonists & inhibitors, metabolism*
Sea Urchins / physiology*
|ZIA DE000712-07/DE/NIDCR NIH HHS|
|0/Dopamine Agonists; 0/Dopamine Antagonists; 0/Receptors, Dopamine D2; VTD58H1Z2X/Dopamine|
Journal ID (nlm-journal-id): 101528555
Journal ID (pubmed-jr-id): 37539
Journal ID (nlm-ta): Nat Commun
Journal ID (iso-abbrev): Nat Commun
nihms-submitted publication date: Day: 8 Month: 4 Year: 2014
Electronic publication date: Day: 20 Month: 12 Year: 2011
pmc-release publication date: Day: 21 Month: 4 Year: 2014
Volume: 2First Page: 592 Last Page: 592
PubMed Id: 22186888
|Rapid Adaptation to Food Availability by a Dopamine-Mediated Morphogenetic Response|
|Diane K. Adams1|
|Mary A. Sewell2|
|Robert C. Angerer1|
|Lynne M. Angerer1|
1 National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892 USA
2 School of Biological Sciences, University of Auckland, Auckland 1142, New Zealand
|Correspondence: Corresponding author: Diane K Adams National Institute of Dental and Craniofacial Research, National Institutes of Health, 30 Convent Drive, Bldg 30 Rm 523, Bethesda, MD 20892 USA firstname.lastname@example.org, Phone: +1 (301) 496-1392, Fax: +1 (301) 480-5353
Predator-prey interactions can produce developmental and morphological responses with significant ecological and evolutionary implications1-5. Predator-induced defenses reduce the prey's risk of consumption, while prey-induced offenses improve consumption or competitive ability in the predators1-3, 6-8. These phenotypic changes can thus affect trophic interactions, predator and prey population dynamics, community dynamics, and can drive speciation1, 2, 6. A mechanistic understanding of these responses is necessary to understand their evolution, limitations, adaptive value, and functional consequences4, 9, 10, but molecular mechanisms remain elusive for prey-induced offenses and all but a few models of predator-induced defenses11, 12.
Echinoid larvae exhibit phenotypic plasticity in feeding structure size after feeding is initiated13, 14 and also in advance of feeding15, providing a tractable developmental model to investigate prey-induced phenotypic plasticity. When food is plentiful, larvae develop smaller feeding arms, whereas when food is scarce, they develop longer feeding arms. Like many other prey-induced responses6, 7, the sea urchin larval response has been described as an offensive response, developing a larger feeding structure when food is scarce in order to improve food acquisition15. Such a response, if adaptive, would allow larvae to adjust to the inherent spatial and temporal variability in their food (algae) supply. It might also allow them to buffer trophic mismatches due to climate change16, 17, as the seasonal peak abundance of some echinoderm larvae in the water column has shifted forward in time16. However, the adaptive value and offensive nature of this prey-induced phenotypic response has not been established. Sea urchin larvae may be able to induce the development of longer arms when food is scarce, the assumed offensive response; alternatively, larvae may only be able to inhibit development from a pre-determined maximum to make shorter arms when food is abundant. The alternative hypotheses result in the same observed phenotypes but through different directional mechanisms with very different organismal (e.g. offensive vs. protective/defensive) and ecological (increasing vs. decreasing grazing/predation pressure) consequences and thus different limitations and implications for selection and evolution.
In order to distinguish whether long arms are induced in low food density or, conversely, whether short arms are induced at high food density, it is necessary to determine the underlying food-response mechanism. In pre-feeding Strongylocentrotus purpuratus larvae, this developmental response occurs over only a few days and requires direct chemosensation of algae15; thus it is likely to occur through a neurosensory mechanism. We report that dopamine is the neuro-signaling pathway by which algal sensation alters development. Dopamine signaling promotes shortening of larval arm length from a developmentally-determined maximum. Thus, unexpectedly, this prey-induced plasticity is not an offensive-response to improve resource acquisition. By experimentally manipulating dopamine signaling and arm length, we assess the costs, benefits, and limitations of this phenotypic response and provide evidence to suggest that sea urchin larva may have co-opted a widely used signaling pathway of food intake for developmental responses.
By screening pharmaceuticals targeted to known sea urchin neurotransmitters, we discovered that dopamine signaling is involved in regulating post-oral arm growth in pre-feeding larvae. We used the length of the skeletal element of the post-oral arms as a proxy for the size of the feeding apparatus (Fig. 1a,b), as this skeletal structure supports the ciliary band with which the larvae capture food particles. As in previous studies15, the response to food was manifested as plasticity in post-oral arm length (Fig. 1c) rather than global changes in body size (Fig. 1d). Thus, body rod length was used here as a control for whole-embryo or non-specific responses to perturbations, such as sickness. Pharmacological activation of dopamine type-D2 receptor (DRD2) function with the agonist, quinpirole, inhibited arm elongation, recapitulating the phenotype observed in larvae reared in the presence of abundant food. Post-oral arm length decreased preferentially compared to body rod length with increasing dosage of quinpirole (Fig. 2a) providing evidence that the direction of the response may be to inhibit growth of the feeding arms. Furthermore, the short-arm phenotypic response to food was suppressed by antagonizing DRD2 with amisulpride (Fig. 2b, Supplementary Table S1), suggesting that dopamine functions downstream of algal sensation.
For an independent test of involvement of the dopamine pathway in algal sensation, we disrupted tyrosine hydroxylase (TH) production and blocked TH activity to prevent dopamine biosynthesis (Fig. 3, Supplementary Table S2, S3). TH is required for the conversion of L-tyrosine to L-DOPA, the dopamine precursor, and is the rate-limiting enzyme in catecholamine biosynthesis. Microinjection of a translation-blocking morpholino antisense oligonucleotide (MASO) targeted to TH (TH MASO1), suppressed the post-oral arm response to algae (Fig. 3a). TH MASO1 morphants developed normally (Fig. 3a, Supplementary Fig. S1, Supplementary Table S2), but lacked detectable TH protein (Fig. 3b). A second TH morpholino (Fig. 3c) as well as pharmaceutical inhibition of TH with α-methyl-p-tyrosine (Fig. 3d) also suppressed the short-arm response to food, confirming the TH MASO1 morphant phenotype.
The responses to pharmaceutical and gene knock-down perturbations of DRD2 and TH were specific, affecting the length of the post-oral arms, but not that of body rods, indicating a local response rather than a non-specific, whole-embryo response (Fig. 2, Fig. 3, Supplementary Fig. S1, Supplementary Table S1-S3). These data suggest that dopamine signaling through a dopamine type-D2 receptor is both sufficient to mimic the response to high algal concentrations and necessary to mediate it.
Dopaminergic neurons develop in the tips of the post-oral arms at the appropriate time to mediate the short-arm response to algae. Cells expressing TH were first detected by immunostaining at the prism stage (Fig. 4a,b) at the onset of elongation of the post-oral arms (Fig. 4c, black arrow), and persisted through embryonic skeletal elongation to the start of feeding (Fig. 4d,e). Dopamine, TH protein, and dopa decarboxylase mRNA were each detected in cells near the post-oral arm tips of plutei, in close proximity to the overlying ectoderm and to the primary mesenchyme cells (PMCs) that fabricate the larval skeleton (Fig. 4d-f). TH-positive cells also express the neural marker, Synaptotagmin B18 (Supplementary Fig. S2), and extend processes along the length of the ciliary band (Fig. 4e), which could be involved in algal sensation. These data are consistent with the distribution of dopamine-positive cells in S. droebachiensis larvae19 and a neurosensory function. Higher magnification (40x) of embryos double-stained for dopamine and a PMC marker revealed that dopamine-positive cells were immediately adjacent to the PMCs (Fig. 4g-j). Although plastic phenotypic responses often involve neuro-stimulation of a secondary endocrine signal9, the close proximity of dopamine-positive cells to the PMCs and overlying ectoderm in the post-oral arms raises the possibility that dopamine may signal directly to one or both cell types to inhibit arm growth.
Gene knock-down (Fig. 3) and pharmaceutical perturbation experiments (Fig. 2, 3) targeting two independent points in the signaling pathway, together with the temporal and spatial expression patterns of dopamine and dopamine biosynthetic enzymes (Fig. 4), identify dopamine as a key signal mediating the phenotypic response to food availability. In the absence of dopamine signaling, plasticity in arm length is lost. Thus the direction of the phenotypic plasticity is clear; short arms are induced at high food density when dopamine signaling inhibits skeletal elongation and thus feeding structure growth.
Showing the involvement of dopamine signaling established that the response is directed toward reducing the size of feeding arms and also allowed us to artificially manipulate the size of the feeding structure to determine the adaptive capacity. We tested the functional consequences for feeding and energy expenditure by pharmaceutically varying feeding apparatus size. This method was advantageous over varying algal concentrations because sea urchin larvae can incorporate exogenous dissolved organic material20, 21 that could change their metabolic state, even though they are not yet feeding.
Previous studies have shown that the size of the feeding apparatus correlates well with the maximum food clearance rate over developmental time13, 22 and among different species23 of echinoderm larvae. The differences in size are greater between species or development stages (e.g. 4-arm vs. 8-arm echinoplutei) than we have observed here at a single developmental stage in a single species. To determine whether the relatively small changes in feeding structure could significantly affect algal capture rates, we pharmaceutically manipulated feeding structure size and then allowed the larvae to feed on a suspension of algal mimics. Variation in post-oral arm length was significantly correlated with (P < 0.001) and explained approximately twenty percent of the variation in bead ingestion rate at low densities (1,500 beads ml−1; Fig. 5a) and more than twenty-five percent of the variation at higher densities (4,000 beads ml−1; Supplementary Fig. S3). The clearance rate increased 8.7% (± 1.2% S.D.) to 11.2% (± 3.4% S.D.) per 10% increase in post-oral arm length. Thus, differential arm elongation during the pre-feeding stage can alter individual fitness by varying the ciliary feeding capacity, and thus potential food intake rate. These, together with the dopamine perturbation results, lead to the conclusion that food induces a rapid morphogenetic response in pre-feeding sea urchin larvae that reduces food intake potential.
The existence of a mechanism to suppress arm growth in high food densities despite the feeding advantage of longer arms seems counterintuitive and suggests that there must be a substantial cost to the long armed phenotype of the pre-feeding larva. To test this idea, we next analyzed the expenditure of maternal triglyceride stores over a range of pharmaceutically induced post-oral arm lengths. Triglycerides are the primary energetic lipids used during pre-feeding development to fuel construction of the feeding larva24, 25. The amount of stored triglycerides, as a percentage of the initial maternal triglyceride investment, was significantly negatively correlated with post-oral arm length at the end of the pre-feeding stage, 5 days post-fertilization (pf) (Fig. 5b). The larval culture with the shortest arms at 5 days pf had 62.0% (± 0.7% SEM) of the initial triglycerides remaining compared to 44.6% (± 2.2% SEM) for the larval culture with the longest arms at 5 days pf (Fig. 5b). Thus, embryos could variably conserve ~15% of the maternal triglyceride load when dopamine signaling was strongly stimulated and larval arms were short. Assuming an average usage of approximately 1 ng triglyceride per individual per day (Supplementary Fig. S4), a savings of 15% represents more than one day of energy. This is a substantial cost early in larval life, as triglycerides are depleted by day 10 (Supplementary Fig. S4) and are not replenished by feeding25. Developmental plasticity through dopamine signaling thus allows larvae to preserve a considerable proportion of maternal energetic reserves.
The food response of sea urchin larvae, like many prey-induced traits, has been thought to be offensive and act to increase predator food acquisition2, 15. In contrast, our data demonstrate that the molecular mechanism actually acts to reduce the size of the feeding structure when algae are detected. The algae-induced dopamine signaling suppresses the developmental ‘default’ program operating in pre-feeding larvae to produce shorter arms with lower food acquisition potential.
For phenotypic plasticity to be maintained, phenotypic changes should maximize fitness for the given environment. We show that the long-armed phenotype maximizes fitness when food is scarce through increased food acquisition potential at the expense of maternal lipids; whereas the short-armed phenotype maximizes fitness when food is abundant through conserving maternal lipids at the expense of maximum food acquisition potential. There may be additional fitness trade-offs, for example between arm length and stomach size26 and/or swimming performance27 associated with this developmental response.
While the functional tradeoffs are bidirectional, the mechanism that establishes the phenotypes is a unidirectional response. Maximum food acquisition rates are the ‘default’ state constrained by the developmental program and/or maternal inputs. Thus, increasing the size of the feeding apparatus beyond its current maximum, to compensate for potential decreases in food resources due to climate change, would require additional maternal investment and/or evolution of the developmental program. The dopamine-signaling mechanism underlying the developmental plasticity is only induced when food is present, and increases in significance when food is abundant. Thus, natural selection has favored the existence of two programs regulating larval arm growth: a developmental program that optimizes food acquisition potential and another that confers developmental plasticity by adjusting the operation of this program to, in part, optimize use of energy stores. An interpretation of this developmental response mechanism might be that there is a finite or diminishing return for increased ciliary feeding at high food concentrations, possibly due to physiological constraints26. However, benefits of the short-armed phenotype, which is induced by food through dopamine signaling, could be a driving factor whether or not ciliary feeding capacity (arm length) is limited.
The ability to allocate maternal energy stores to other purposes could have substantial consequences for survival and performance success across multiple life-history stages through latent effects28-30. For example, through increased allocation towards development of the juvenile, larvae might be able to decrease the length of time they are exposed to predators in the plankton, or there might be an increase in the quality of the settled juveniles29, 30. Since S. purpuratus has a high post-settlement mortality rate with infrequent successful recruitment events31, 32, condition at settlement could be an important factor controlling the dynamics of benthic sea urchin populations, which have community level consequences33,34. If mismatches between larval abundances and food availability increase in frequency or severity, the reduced algal abundances during the pre-feeding stage would result in an increased occurrence of long-armed larvae and a loss of any benefits of short-armed larvae, such as reallocation of maternal energy stores.
Our data demonstrate the adaptive use of dopamine signaling to rapidly alter development of feeding structures in anticipation of food. Only by gaining a mechanistic understanding could this prey-induced change in form be placed in the appropriate ecological and also evolutionary context. Diverse organisms, from worms to humans, use dopamine signaling35, 36, through dopamine type-D2 receptors in particular 37, 38, to anticipate food consumption. However, in C. elegans35, 38, insects39, 40, adult sea urchins41, and mammals, including humans36, dopamine signaling alters behavior in anticipation of food, whereas pre-feeding sea urchin larvae alter their development and form, as presented here. Thus, the dopamine signaling involved in regulating larval development may represent a co-option or redeployment of the highly conserved use of dopamine signaling in mediating behavior in anticipation of food.
Adult sea urchins, Strongylocentrotus purpuratus, from Cultured Abalone, Goleta, CA and the Point Loma Marine Invertebrate Lab, CalTech, CA were maintained in seawater at 10°C. Gametes were harvested via intracoelomic injections of 0.55 M KCl. Embryos were cultured using standard methods at densities of 1-5 embryos ml−1 in artificial seawater (ASW) at 15°C, with or without algae (~5,000 cells ml−1 of Dunaliella sp.).
Post-oral arm and body rod lengths (n ≥ 20, each) were assayed from a random sample taken at 5 days pf, just before feeding begins, except for morpholino-injected embryos which were sampled at 4 days pf to ensure that morpholino knockdown of TH protein synthesis was still effective. Larvae were squash mounted on microscope slides to position the skeletal elements in the same plane, then imaged on a Zeiss Axiovert 200M inverted microscope at 20X under differential inference contrast which readily identifies the birefringent skeletal elements. The skeletal lengths were quantified from the digital images using AxioVision software (Carl Zeiss MicroImaging). In some cases (< 1%), a skeletal element could not be measured due to breakage or exit from the plane of focus.
Dopamine signaling was perturbed using quinpirole, a widely used and specific type-D2 receptor agonist42, amisulpride, a highly selective D2/D3 dopamine receptor antagonist43, and α-Methyl-DL-tyrosine methyl ester hydrochloride, a selective inhibitor of TH44, administered directly via dilution into the culture medium (ASW) to full-sibling larvae at late gastrula stage (48-52 hpf), unless otherwise noted in the text. Pharmaceutical experiments were performed in duplicate with different female-male pairings.
Fertilized eggs were microinjected with filtered solutions containing 20% glycerol and rhodamine-dextran without morpholinos (glycerol control), with 0.4 mM TH MASO1, or with 0.15 mM TH MASO2 using an Eppendorf Femtojet-Injectman NI2 micromanipulator attached to a Leica inverted fluorescent microscope, as in 45. TH MASO1 injections were replicated with two different female-male pairings. Glycerol and uninjected embryos were compared and no significant changes in skeletal lengths, TH immunostaining, or response to algae were detected; thus, some later experiments used uninjected embryos as controls. The translation-blocking TH MASO1 was designed based on the predicted tyrosine hydroxylase (GLEAN 00836) 5’UTR, which was confirmed by RACE. TH MASO1: 5’-GCGTCCTGCTGTAGAAGATACTTGA-3’. TH MASO2 targeted the intron 8/exon 9 boundary to disrupt the catalytic domain of the enzyme46. TH MASO2: 5’- GCCTACGATGAACAAGAACAAATGT-3’.
The dose-response of post-oral arm length to treatment with quinpirole was assessed using a one-way ANOVA and subsequent Bonferroni-corrected pair-wise comparisons versus the control to determine if the change relative to control was significant. The response to algae was assessed in pharmaceutical and TH MASO1 perturbations by a three-way ANOVA where perturbation treatment, algal treatment (with vs. without algae), and experimental replicate were fixed effects and the mean square error (MSE) was used as the denominator of the f-ratio47. Since all experiments showed a significant (P > 0.05) interaction between perturbation and algal treatments for post-oral arm length, subsequent Bonferroni-corrected pair-wise comparisons were performed to determine if the algae-induced change was significant. TH MASO2 was not replicated and was thus assessed using Student's two-tailed t-tests. All statistical analyses were done in SYSTAT v10 with output to three decimal places, thus exact P values are given if P > 0.001.
Immunostains for dopamine (1:1500, ab6428, Abcam), Tyrosine Hydroxylase (1:200, ImmunoStar), Msp130 (1:5, 6a9)48, SM30 (1:400, 3814, courtesy of F.H. Wilt)49, and Synaptotagmin B (1:500, rat, courtesy of R. Burke) were performed as in 50. To determine the time of initial expression of TH, embryos were incubated in the TH antibody at 4°C over two nights to improve sensitivity. Fluorescent whole-mount in situ hybridization for dopa decarboxylase was carried out as in 45, 51, except for inclusion of two more stringent post-hybridization washes in 70% hybridization buffer for 45 min each. Two fragments (470 bp and 758 bp) of the dopa decarboxylase gene isolated with primers: forward1, 5’-CTCCGATGATTTCCGTGTCT-3’; reverse1, 5’-CTGCCAGCAAGGCTACTAGA-3’; forward2, 5’-CCACCGATGACAAAGGTTCT-3’; and reverse2, 5’-CCAGGCATACGTCATGTGTC-3’ were used to generate probes used simultaneously to detect gene expression. Embryos were imaged using a Zeiss Axiovert 200M inverted microscope at 20X or 40X. Optical sections were obtained with an ApoTome unit (Zeiss, Thornwood, NY) and stacked images were prepared using Imaris (Bitplane Inc., St. Paul, MN).
We treated half-sib larvae from three females with artificial seawater (control), DRD2 agonist (quinpirole) and DRD2 antagonist (amisulpride) to maximize the size range of the feeding structures. Larvae from each of these treatments were combined into three replicate cultures, transferred to ASW for four hours, and then allowed to feed on a gently stirred suspension of algal mimics, 10 μm Polybead ® Microspheres (Polysciences, Inc) coated with BSA at 1,500 or 4,000 beads ml−1, for 45 minutes. The number of beads ingested and skeletal lengths were measured for at least 30 larvae from each replicate as above. There was no significant difference between replicate samples fed at 1,500 beads ml−1 (two-factor ANOVA, two-tailed P = 0.950, SYSTAT v.10), so the replicates were pooled and analyzed using a model I regression.
Duplicate cultures, each consisting of half-sibs from three females, were cultured in gently stirred 500 ml beakers within a 15°C incubator. At the onset of arm elongation (~65 hpf), the cultures were divided into three treatments: control, amisulpride 25 μM and quinpirole 50 μM. Replicate samples of 250 individuals each were collected from each treatment on days 0, 2, 3, 4, 5, 6, 8, and 10 pf and stored frozen at −80 °C until lipid analysis using an Iatroscan Mark 5new TLC/FID (flame ionization detection). Lipid was extracted from frozen embryo and larval samples with ketone as an internal standard24. Immediately before spotting, the lipid extract was dried in a stream of N2 gas and 10 μl of HPLC-grade chloroform was added using a Gilson positive displacement pipette. The rack containing 10 Chromarods was positioned on a warm hot-plate during spotting of 5 μl of the lipid extract onto 8 individual Chromarods to ensure that the sample remained in a narrow band at the origin. The remaining two Chromarods were used to run an unspotted negative control24 and a composite lipid standard to confirm peak identification and prepare standard curves for quantification of the 8 lipid classes24 plus diglyceride. The Chromarods were run in a double development: Hexane:Diethyl Ether:Formic acid (98.5:1:0.05) for two sequential developments of 25 and 20 min followed by 5 min drying at 60°C and a partial scan; 36 minutes in Hexane:Diethyl Ether:Formic acid (79:20:1), drying and a full scan. We present triglyceride concentrations for each treatment and time-point based on lipid extracts obtained from three replicate samples, each containing 250 individuals, as a measurement of maternal energetic stores.
Normalized, mean post-oral arm length and triglyceride levels were assessed using a Model II geometric mean regression52 to take into account the error associated with both variables. Post-oral arm length was normalized to the maximum. Triglyceride concentration was normalized to the initial concentration in fertilized eggs (day 0, pre-cleavage). Treatments within a replicate culture had the same initial triglyceride concentrations, allowing for direct comparisons between treatments. Since there was no interaction with cultures (two-factor ANOVA, two-tailed P = 0.478, SYSTAT v.10), the two cultures were pooled for the regression.
FN1Author contributions D.K.A. led the design and execution of the experiments, data analyses, and manuscript preparation. M.A.S. contributed the lipid analysis design and execution. L.M.A. and R.C.A. contributed to the experimental design and data analyses. All authors contributed to manuscript preparation.
FN2The authors declare no competing financial interests.
Supporting Information includes Supplementary Figures S1-S4 and Supplementary Tables S1-S3.
We are grateful for discussions with R. Range, A. Sethi, and Z. Wei. Support was provided by the Division of Intramural Research in the National Institute for Dental and Craniofacial Research, National Institutes of Health and the University of Auckland.
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