Document Detail

Rapid, accurate and non-invasive detection of cerebrospinal fluid leakage using combined determination of beta-trace protein in secretion and serum.
MedLine Citation:
PMID:  15563887     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: beta-Trace protein (Btp) has been proposed as a valuable marker of cerebrospinal fluid (CSF) leakage overcoming the drawbacks of beta-2-transferrin (B-2Tr) determination. However, there is still controversy about the appropriate cut-offs to be used (range 0.35-6 mg/L). The aim of the study was to evaluate cut-offs of Btp determination for detection CSF leakage. Further, we assessed whether the Btp secretion to serum ratio (Btp-sec/ser-ratio) would add diagnostic value. METHODS: Prospective study in patients with suspected CSF leakage. Quantitative determination of Btp in secretion and serum (Dade-Behring) and qualitative measurement of B-2-Tr in secretion and serum. Results were assessed in view of clinical data. Cut-offs and diagnostic characteristics were determined by ROC analysis. RESULTS: A total of 176 samples were assessed originating from 105 patients. In 43 samples CSF leakage could be confirmed. Sensitivity of B-2-Tr was 84%, specificity amounted to 100%. The area under the curve (AUC) for Btp-measurement in secretion was 0.98. At a cut-off of 0.68 mg/L, sensitivity was 100% and specificity 91%. At a cut-off of 1.11 mg/L, the specificity was 100% with a sensitivity of 93%. The Btp-sec/ser-ratio has an AUC of 0.99. Combining a 0.68 mg/L cut-off in secretion with a Btp-sec/ser-ratio cut-off of 4.9 reveals a sensitivity of 99% and a specificity of 100%. CONCLUSIONS: Btp is a rapid and accurate marker for the presence of CSF leakage. Combining measurement of Btp in secretion together with determination of the Btp-sec/ser-ratio enhances the diagnostic characteristics of the Btp assay. Determination of Btp in both serum and secretion is thus recommended.
Lorenz Risch; Ivan Lisec; Markus Jutzi; Michael Podvinec; Hans Landolt; Andreas R Huber
Related Documents :
6452817 - Immunosuppressive activity of rhesus monkey pregnancy serum.
3593767 - Properties and serum levels of pregnancy-associated variant of human transcortin.
9600497 - Eosinophilia and gastrointestinal symptoms after ingestion of shiitake mushrooms.
7409607 - First-trimester serum prolactin levels in normal and complicated pregnancies.
22365957 - Implications of maternal nutrient restriction in transgenerational programming of hyper...
10392797 - Constant multiple birth rates in the czech republic and the slovak republic until recen...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinica chimica acta; international journal of clinical chemistry     Volume:  351     ISSN:  0009-8981     ISO Abbreviation:  Clin. Chim. Acta     Publication Date:  2005 Jan 
Date Detail:
Created Date:  2004-11-26     Completed Date:  2005-04-18     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  1302422     Medline TA:  Clin Chim Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  169-76     Citation Subset:  IM    
Clinical Decision Making Research Unit, Vorarlberg Institute of Vascular Investigation and Treatment, Academic Teaching Hospital, Feldkirch, Austria.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Biological Markers
Cerebrospinal Fluid / physiology*
Craniocerebral Trauma / cerebrospinal fluid*
Ear / injuries
Intramolecular Oxidoreductases / blood,  cerebrospinal fluid*
Middle Aged
Nephelometry and Turbidimetry
Neurosurgical Procedures
Nose / injuries
Prospective Studies
ROC Curve
Skull Fractures / cerebrospinal fluid,  radiography
Transferrin / cerebrospinal fluid
Reg. No./Substance:
0/Biological Markers; 0/Lipocalins; 11096-37-0/Transferrin; EC 5.3.-/Intramolecular Oxidoreductases; EC R2 D-isomerase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Relationship of serum leptin with age, body weight, body mass index, and bone mineral density in hea...
Next Document:  Preeclampsia-associated reduction of cathepsin D activity in the umbilical cord.