Document Detail


Rapid Shotgun Proteomic LC-ESI-MS/MS-based Method for the Lupin (Lupinus albus L.) Multi-Allergen Determination in Foods.
MedLine Citation:
PMID:  22612429     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Allergy to lupin is a growing food safety problem since this legume, increasingly exploited in food industry, is one of the allergens that according to law must be declared on the labels of food products in the European Union. In this context, a rapid targeted proteomic approach based on liquid chromatography-electrospray-tandem mass spectrometry (LC-ESI-MS/MS) analysis was proposed and aimed to unequivocal confirmation and reliable determination of the major lupin allergens, i.e conglutins, in pasta and biscuits. Detected concentrations were around 1 mg lupin/kg pasta and biscuits, proving the capabilities of the MS-based method in terms of sensitive allergen screening method. Good precision was observed both in terms of intra-day and inter-day repeatability with RSD lower than 23%. Recoveries in the 95(±10)-118(±12)% and 103(±1)-110(±12)% ranges were calculated for biscuits and pasta, respectively. Finally, the applicability of the devised method was investigated by analyzing market samples containing lupin and samples that may possibly contain traces of lupin deriving from cross contamination between products and production lines.
Authors:
Monica Mattarozzi; Chiara Bignardi; Lisa Elviri; Maria Careri
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-5-21
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  -     ISSN:  1520-5118     ISO Abbreviation:  -     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-5-22     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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