| Rapid separation and quantification of abscisic Acid from plant tissues using high performance liquid chromatography. | |
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MedLine Citation:
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PMID: 16659949 Owner: NLM Status: PubMed-not-MEDLINE |
Abstract/OtherAbstract:
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Abscisic acid (ABA) was purified from soybean (Glycine max [L.]) seed extract using a preparative high performance liquid chromatography (HPLC) procedure. The preparative procedure was rapid (70 minutes per sample), required no prior partitioning for purification and was quantitative as demonstrated with an internal standard of [2-(14)C]ABA, of which 98.9% was recovered.Following purification by the preparative HPLC procedure, the ABA in a soybean seed extract was quantified using either GLC with an electron capture detector (GLC-EC) or by analytical HPLC with a UV detector. For soybean seed extracts, two analytical HPLC column packing materials were found adequate: muPorasil and muBondapak-NH(2) (Waters Associates). However, with complex tissue extracts, such as soybean leaf and nodule tissues, only GLC-EC had the necessary selectivity and sensitivity. |
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Authors:
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A J Ciha; M L Brenner; W A Brun |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Plant physiology Volume: 59 ISSN: 0032-0889 ISO Abbreviation: Plant Physiol. Publication Date: 1977 May |
Date Detail:
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Created Date: 2010-06-29 Completed Date: 2010-06-29 Revised Date: 2010-09-14 |
Medline Journal Info:
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Nlm Unique ID: 0401224 Medline TA: Plant Physiol Country: United States |
Other Details:
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Languages: eng Pagination: 821-6 Citation Subset: - |
Affiliation:
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University of Minnesota, St. Paul, Minnesota 55108. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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