| Rapid PCR detection of the methicillin resistance gene, mecA, on the hands of medical and non-medical personnel and healthy children and on surfaces in a neonatal intensive care unit. | |
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MedLine Citation:
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PMID: 11515757 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The hands of medical personnel are the chief vectors for transmission of antibiotic-resistant bacteria and probably serve as an important reservoir for antibiotic resistance genes in hospitals. In this survey we examined different reservoirs of the methicillin resistance gene, mecA, using a simplified PCR method. Samples (n = 151) were taken from the hands of medical and non-medical personnel and healthy children and from surfaces in a neonatal intensive care unit (NICU). We also performed sampling from 4 different body sites in 5 of the medical personnel. Fifteen out of 16 nurses (94%) from the ICU carried the mecA gene on their hands, whereas only 35% of the paediatric nurses were mecA-positive. Of all medical personnel, 44% carried the mecA gene on their hands. There was a significant difference (p < 0.015) between medical and non-medical personnel in terms of the carriage rate of mecA. Four samples from surfaces in a NICU--2 ventilators, 1 bench and 1 telephone--were positive for mecA. Our results are comparable with those from previous studies on reservoirs of methicillin-resistant coagulase-negative staphylococci using conventional culture techniques. |
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Authors:
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C Klingenberg; G T Glad; R Olsvik; T Flaegstad |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Scandinavian journal of infectious diseases Volume: 33 ISSN: 0036-5548 ISO Abbreviation: Scand. J. Infect. Dis. Publication Date: 2001 |
Date Detail:
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Created Date: 2001-08-22 Completed Date: 2002-01-15 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0215333 Medline TA: Scand J Infect Dis Country: Sweden |
Other Details:
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Languages: eng Pagination: 494-7 Citation Subset: IM |
Affiliation:
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Department of Paediatrics, University Hospital Tromsø, Norway. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Bacterial Proteins* Carrier Proteins / genetics* Child Child, Preschool Environmental Microbiology* Hand / microbiology* Hexosyltransferases* Humans Infant Infant, Newborn Infectious Disease Transmission, Professional-to-Patient Intensive Care Units, Neonatal Interior Design and Furnishings* Methicillin Resistance / genetics* Muramoylpentapeptide Carboxypeptidase / genetics* Penicillin-Binding Proteins Peptidyl Transferases* Personnel, Hospital* Polymerase Chain Reaction / methods* Staphylococcal Infections / genetics, transmission* |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Carrier Proteins; 0/Penicillin-Binding Proteins; EC 2.3.2.12/Peptidyl Transferases; EC 2.4.1.-/Hexosyltransferases; EC 3.4.17.8/Muramoylpentapeptide Carboxypeptidase |
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