Document Detail


Rapid, massively parallel single-cell drug response measurements via live cell interferometry.
MedLine Citation:
PMID:  21889438     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A central question in cancer therapy is how individual cells within a population of tumor cells respond to drugs designed to arrest their growth. However, the absolute growth of cells, their change in physical mass, whether cancerous or physiologic, is difficult to measure directly with traditional techniques. Here, we develop live cell interferometry for rapid, real-time quantification of cell mass in cells exposed to a changing environment. We used tunicamycin induction of the unfolded protein stress response in multiple myeloma cells to generate a mass response that was temporally profiled for hundreds of cells simultaneously. Within 2 h, the treated cells were growth suppressed compared to controls, with a few cells in both populations showing a robust increase (+15%) or little change (<5%) in mass accumulation. Overall, live cell interferometry provides a conceptual advance for assessing cell populations to identify, monitor, and measure single cell responses, such as to therapeutic drugs.
Authors:
Jason Reed; Jennifer Chun; Thomas A Zangle; Sheraz Kalim; Jason S Hong; Sarah E Pefley; Xin Zheng; James K Gimzewski; Michael A Teitell
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biophysical journal     Volume:  101     ISSN:  1542-0086     ISO Abbreviation:  Biophys. J.     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-09-05     Completed Date:  2011-12-23     Revised Date:  2014-09-13    
Medline Journal Info:
Nlm Unique ID:  0370626     Medline TA:  Biophys J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1025-31     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Biophysical Society. Published by Elsevier Inc. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Cell Adhesion / drug effects
Cell Division / drug effects
Cell Line, Tumor
Cell Survival
Humans
Interferometry / methods*
Single-Cell Analysis / methods*
Time Factors
Tunicamycin / pharmacology*
Unfolded Protein Response / drug effects
Grant Support
ID/Acronym/Agency:
PN2EY018228/EY/NEI NIH HHS; R01 CA090571/CA/NCI NIH HHS; R01 CA156674/CA/NCI NIH HHS; R21GM074509/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
11089-65-9/Tunicamycin
Comments/Corrections

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