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Rapid Isolation and Purification of Inotodiol and Trametenolic Acid from Inonotus obliquus by High-speed Counter-current Chromatography with Evaporative Light Scatting Detection.
MedLine Citation:
PMID:  21433158     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Introduction - In Eastern Europe, especially Russia, the fruiting body of Inonotus obliquus has been used as a folk medicine for cancer since the sixteenth or seventeenth century. Inotodiol and trametenolic acid are considered to be the main bioactive compounds of the fruiting body of the mushroom. These compounds show various biological activities, including anti-tumour, anti-viral, hypoglycaemic, anti-oxidant and cyto-protective. However, effective methods for isolating and purifying inotodiol and trametenolic acid from the fruiting body of Inonotus obliquus are not currently available. Objective - To develop a suitable preparative method in order to isolate inotodiol and trametenolic acid from a complex Inonotus obliquus extract by preparative high-speed counter-current chromatography (HSCCC). Methodology - Inotodiol and trametenolic acid were rapidly isolated and purified from the chloroform extract of Inonotus obliquus (Fr.) by HSCCC with evaporative light scatting detection (ELSD). The purity of the obtained target compounds was analysed by high-performance liquid chromatography (HPLC) with ELSD. The structures of the two compounds were identified by (1) H NMR and (13) C NMR. Result - The target compounds were finally isolated and purified with a solvent system of hexane:ethyl acetate:methanol:water (1:0.4:1:0.4, v/v/v/v). In a single operation, 100 mg of the I. obliquus extracts yielded 13.0 mg of inotodiol and 7.0 mg of trametenolic acid. The entire separation and purification process took less than 5 h. The purities of obtained inotodiol and trametenolic acid were 97.51 and 94.04%, respectively. Conclusion - HSCCC-ELSD was an efficient and rapid method for the separation and purification of inotodiol and trametenolic acid from I. obliquus. Copyright © 2011 John Wiley & Sons, Ltd.
Authors:
Deyao Du; Feng Zhu; Xianhui Chen; Xiuyun Ju; Youjian Feng; Lian-Wen Qi; Jihong Jiang
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-3-24
Journal Detail:
Title:  Phytochemical analysis : PCA     Volume:  -     ISSN:  1099-1565     ISO Abbreviation:  -     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-3-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9200492     Medline TA:  Phytochem Anal     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 John Wiley & Sons, Ltd.
Affiliation:
Key Laboratory for Biotechnology of Medicinal Plant of Jiangsu Province, Xuzhou Normal University, Xuzhou, 221116, China.
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