| Rapid Isolation and Purification of Inotodiol and Trametenolic Acid from Inonotus obliquus by High-speed Counter-current Chromatography with Evaporative Light Scatting Detection. | |
| | |
MedLine Citation:
|
PMID: 21433158 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Introduction - In Eastern Europe, especially Russia, the fruiting body of Inonotus obliquus has been used as a folk medicine for cancer since the sixteenth or seventeenth century. Inotodiol and trametenolic acid are considered to be the main bioactive compounds of the fruiting body of the mushroom. These compounds show various biological activities, including anti-tumour, anti-viral, hypoglycaemic, anti-oxidant and cyto-protective. However, effective methods for isolating and purifying inotodiol and trametenolic acid from the fruiting body of Inonotus obliquus are not currently available. Objective - To develop a suitable preparative method in order to isolate inotodiol and trametenolic acid from a complex Inonotus obliquus extract by preparative high-speed counter-current chromatography (HSCCC). Methodology - Inotodiol and trametenolic acid were rapidly isolated and purified from the chloroform extract of Inonotus obliquus (Fr.) by HSCCC with evaporative light scatting detection (ELSD). The purity of the obtained target compounds was analysed by high-performance liquid chromatography (HPLC) with ELSD. The structures of the two compounds were identified by (1) H NMR and (13) C NMR. Result - The target compounds were finally isolated and purified with a solvent system of hexane:ethyl acetate:methanol:water (1:0.4:1:0.4, v/v/v/v). In a single operation, 100 mg of the I. obliquus extracts yielded 13.0 mg of inotodiol and 7.0 mg of trametenolic acid. The entire separation and purification process took less than 5 h. The purities of obtained inotodiol and trametenolic acid were 97.51 and 94.04%, respectively. Conclusion - HSCCC-ELSD was an efficient and rapid method for the separation and purification of inotodiol and trametenolic acid from I. obliquus. Copyright © 2011 John Wiley & Sons, Ltd. |
| | |
Authors:
|
Deyao Du; Feng Zhu; Xianhui Chen; Xiuyun Ju; Youjian Feng; Lian-Wen Qi; Jihong Jiang |
Related Documents
:
|
3121448 - Homogentisic acid autoxidation and oxygen radical generation: implications for the etio... 7853868 - Effects on anti-lipid peroxidation of cudrania cochinchinensis var. gerontogea. 21292858 - Alanine-scanning mutagenesis of selected residues in the n-terminal region alters the f... 11305258 - Determination of benzoyl peroxide and benzoic acid levels by hplc during wheat flour bl... 7843108 - Complexing of amino acids to dna by chromate in intact cells. 15041028 - Docosahexaenoic acid in the diet: its importance in maintenance and restoration of neur... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-3-24 |
Journal Detail:
|
Title: Phytochemical analysis : PCA Volume: - ISSN: 1099-1565 ISO Abbreviation: - Publication Date: 2011 Mar |
Date Detail:
|
Created Date: 2011-3-24 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9200492 Medline TA: Phytochem Anal Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2011 John Wiley & Sons, Ltd. |
Affiliation:
|
Key Laboratory for Biotechnology of Medicinal Plant of Jiangsu Province, Xuzhou Normal University, Xuzhou, 221116, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Metabolomics Study on Quality Control and Discrimination of Three Curcuma Species based on Gas Chrom...
Next Document: Fast Ultrasound-assisted Extraction of Polar (phenols) and Nonpolar (lipids) Fractions in Heterothec...