Document Detail

Rapid identification and quantification of tumor cells using an electrocatalytic method based on gold nanoparticles.
MedLine Citation:
PMID:  19911778     Owner:  NLM     Status:  MEDLINE    
There is a high demand for simple, rapid, efficient, and user-friendly alternative methods for the detection of cells in general and, in particular, for the detection of cancer cells. A biosensor able to detect cells would be an all-in-one dream device for such applications. The successful integration of nanoparticles into cell detection assays could allow for the development of this novel class of cell sensors. Indeed, their application could well have a great future in diagnostics, as well as other fields. As an example of a novel biosensor, we report here an electrocatalytic device for the specific identification of tumor cells that quantifies gold nanoparticles (AuNPs) coupled with an electrotransducing platform/sensor. Proliferation and adherence of tumor cells are achieved on the electrotransducer/detector, which consists of a mass-produced screen-printed carbon electrode (SPCE). In situ identification/quantification of tumor cells is achieved with a detection limit of 4000 cells per 700 microL of suspension. This novel and selective cell-sensing device is based on the reaction of cell surface proteins with specific antibodies conjugated with AuNPs. Final detection requires only a couple of minutes, taking advantage of the catalytic properties of AuNPs on hydrogen evolution. The proposed detection method does not require the chemical agents used in most existing assays for the detection of AuNPs. It allows for the miniaturization of the system and is much cheaper than other expensive and sophisticated methods used for tumor cell detection. We envisage that this device could operate in a simple way as an immunosensor or DNA sensor. Moreover, it could be used, even by inexperienced staff, for the detection of protein molecules or DNA strands.
Alfredo de la Escosura-Muñiz; Christian Sánchez-Espinel; Belén Díaz-Freitas; Africa González-Fernández; Marisa Maltez-da Costa; Arben Merkoçi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical chemistry     Volume:  81     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-12-16     Completed Date:  2010-02-24     Revised Date:  2012-05-23    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  10268-74     Citation Subset:  IM    
Nanobioelectronics & Biosensors Group, Catalan Institute of Nanotechnology, CIN2 (ICN-CSIC), Campus de la UAB, [corrected] Barcelona, Spain.
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MeSH Terms
Antibodies, Monoclonal / immunology
Antigen-Antibody Reactions
B-Lymphocytes / immunology,  pathology*
Biosensing Techniques*
Carbon / chemistry
Cell Adhesion
Cell Line, Tumor
Cell Proliferation
Gold / chemistry*
HLA-DR Antigens / immunology
Metal Nanoparticles / chemistry*
Prostatic Neoplasms / pathology*
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/HLA-DR Antigens; 7440-44-0/Carbon; 7440-57-5/Gold
Erratum In:
Anal Chem. 2012 Apr 17;84(8):3855

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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