Document Detail

Rapid Identification of Calotropagenin Glycosides Using High-Performance Liquid Chromatography Electrospray Ionisation Tandem Mass Spectrometry.
MedLine Citation:
PMID:  21656605     Owner:  NLM     Status:  Publisher    
INTRODUCTION: Cardiac glycosides in Calotropis procera have therapeutic use as inhibitors of Na(+) /K(+) -ATPase to regulate heart contractions. A large amount of research attention has been received by these compounds towards their identification and structural characterisation. In order to achieve rapid identification of cardiac glycosides in phytochemical extracts a liquid chromatography tandem mass spectrometry (LC-MS/MS) method has been developed involving metal cationisation by post-column addition of alkali salts for the unambiguous determination of their molecular weights. OBJECTIVE: Identification of cardiac glycosides in Calotropis procera leaf extract. RESULTS: Calotropagenin and its 10 glycosides were unambiguously identified. The daughter ions at m/z 387, 369, 359, 351, 341 and 323 in their MS/MS spectra were attributed to the calotropagenin aglycone unit. CONCLUSION: High performance liquid chromatography in combination with electrospray ionization tandem mass spectrometry involving metal cationisation by post column addition of alkali salts was successfully utilised for the rapid identification of calotropagenin glycosides/derivatives in Calotropis procera extract. Copyright © 2011 John Wiley & Sons, Ltd.
Sanjeev Kanojiya; K P Madhusudanan
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-6-7
Journal Detail:
Title:  Phytochemical analysis : PCA     Volume:  -     ISSN:  1099-1565     ISO Abbreviation:  -     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-6-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9200492     Medline TA:  Phytochem Anal     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 John Wiley & Sons, Ltd.
Sophisticated Analytical Instrument Facility, Central Drug Research Institute, Lucknow, 226001, India.
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