Document Detail


Rapid high-resolution mapping of balanced chromosomal rearrangements on tiling CGH arrays.
MedLine Citation:
PMID:  21907824     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The diagnosis and classification of many cancers depends in part on the identification of large-scale genomic aberrations such as chromosomal deletions, duplications, and balanced translocations. Array-based comparative genomic hybridization (array CGH) can detect chromosomal imbalances on a genome-wide scale but cannot reliably identify balanced chromosomal rearrangements. We describe a simple modification of array CGH that enables simultaneous identification of recurrent balanced rearrangements and genomic imbalances on the same microarray. Using custom tiling oligonucleotide arrays and gene-specific linear amplification primers, translocation CGH (tCGH) maps balanced rearrangements to ∼100-base resolution and facilitates the rapid cloning and sequencing of novel rearrangement breakpoints. As proof of principle, we used tCGH to characterize nine of the most common gene fusions in mature B-cell neoplasms and myeloid leukemias. Because tCGH can be performed in any CGH-capable laboratory and can screen for multiple recurrent translocations and genome-wide imbalances, it should be of broad utility in the diagnosis and classification of various types of lymphomas, leukemias, and solid tumors.
Authors:
Harvey A Greisman; Noah G Hoffman; Hye Son Yi
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-09-09
Journal Detail:
Title:  The Journal of molecular diagnostics : JMD     Volume:  13     ISSN:  1943-7811     ISO Abbreviation:  J Mol Diagn     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-10-18     Completed Date:  2012-04-02     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  100893612     Medline TA:  J Mol Diagn     Country:  United States    
Other Details:
Languages:  eng     Pagination:  621-33     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology. Published by Elsevier Inc. All rights reserved.
Affiliation:
Department of Laboratory Medicine, University of Washington, Seattle, WA, USA. hgreisman@gmail.com
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Cell Line, Tumor
Chromosome Aberrations*
Chromosome Deletion
Chromosome Mapping / methods*
Comparative Genomic Hybridization / methods*
Humans
In Situ Hybridization, Fluorescence / methods
Leukemia, Myeloid / diagnosis,  genetics*
Lymphoma / diagnosis,  genetics*
Oligonucleotide Array Sequence Analysis / methods
Polymerase Chain Reaction / methods
Sequence Analysis, DNA
Sequence Deletion
Translocation, Genetic
Grant Support
ID/Acronym/Agency:
5-P30-CA015704/CA/NCI NIH HHS
Comments/Corrections

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