| Rapid detection of lactate dehydrogenase and genotyping of Plasmodium falciparum in saliva of children with acute uncomplicated malaria. | |
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MedLine Citation:
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PMID: 20810809 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The diagnosis of malaria in biological fluids other than blood using non-invasive, rapid diagnostic techniques provides a valuable approach in case management and epidemiological studies of malaria. Rapid detection of Plasmodium falciparum lactate dehydrogenase (pLDH) in saliva samples from 130 of 144 children with microscopically confirmed P. falciparum infection was evaluated using Optimal-IT dipsticks. Genotyping of parasites was also performed in saliva and blood samples from a cohort of patients by polymerase chain reaction (PCR). The sensitivity of the dipstick in whole-blood, whole-saliva, or supernatant of spun saliva samples was 97.2%, 77.9%, and 48.4%, respectively. The sensitivity of the dipstick in whole-saliva samples was significantly higher than in supernatant of spun saliva samples (P < 0.0005). Mutant T76 allele was detectable in 60% and 57% of blood and saliva samples, respectively. This finding shows rapid detection of pLDH in patient saliva. |
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Authors:
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Grace O Gbotosho; Christian T Happi; Onikepe Folarin; Ochuko Keyamo; Akintunde Sowunmi; Ayoade M J Oduola |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The American journal of tropical medicine and hygiene Volume: 83 ISSN: 1476-1645 ISO Abbreviation: Am. J. Trop. Med. Hyg. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-09-02 Completed Date: 2010-09-27 Revised Date: 2011-09-13 |
Medline Journal Info:
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Nlm Unique ID: 0370507 Medline TA: Am J Trop Med Hyg Country: United States |
Other Details:
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Languages: eng Pagination: 496-501 Citation Subset: AIM; IM |
Affiliation:
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Department of Pharmacology and Therapeutics, College of Medicine, University of Ibadan, Ibadan, Nigeria. solagbotosho@yahoo.co.uk |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Child Child, Preschool Genotype Humans L-Lactate Dehydrogenase / analysis* Plasmodium falciparum / enzymology, genetics, isolation & purification* Polymerase Chain Reaction Polymorphism, Genetic Polymorphism, Restriction Fragment Length Saliva / parasitology* |
| Chemical | |
Reg. No./Substance:
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EC 1.1.1.27/L-Lactate Dehydrogenase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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