Document Detail

Rapid Detection and Identification of Yersinia pestis from Food Using Immunomagnetic Separation and Pyrosequencing.
MedLine Citation:
PMID:  23091729     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Interest has recently been renewed in the possible use of Y. pestis, the causative agent of plague, as a biological weapon by terrorists. The vulnerability of food to intentional contamination coupled with reports of humans having acquired plague through eating infected animals that were not adequately cooked or handling of meat from infected animals makes the possible use of Y. pestis in a foodborne bioterrorism attack a reality. Rapid, efficient food sample preparation and detection systems that will help overcome the problem associated with the complexity of the different matrices and also remove any ambiguity in results will enable rapid informed decisions to be made regarding contamination of food with biothreat agents. We have developed a rapid detection assay that combines the use of immunomagnetic separation and pyrosequencing in generating results for the unambiguous identification of Y. pestis from milk (0.9 CFU/mL), bagged salad (1.6 CFU/g), and processed meat (10 CFU/g). The low detection limits demonstrated in this assay provide a novel tool for the rapid detection and confirmation of Y. pestis in food without the need for enrichment. The combined use of the iCropTheBug system and pyrosequencing for efficient capture and detection of Y. pestis is novel and has potential applications in food biodefence.
Kingsley K Amoako; Michael J Shields; Noriko Goji; Chantal Paquet; Matthew C Thomas; Timothy W Janzen; Cesar I Bin Kingombe; Arnold J Kell; Kristen R Hahn
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Publication Detail:
Type:  Journal Article     Date:  2012-10-03
Journal Detail:
Title:  Journal of pathogens     Volume:  2012     ISSN:  2090-3065     ISO Abbreviation:  J Pathog     Publication Date:  2012  
Date Detail:
Created Date:  2012-10-23     Completed Date:  2012-10-24     Revised Date:  2013-04-02    
Medline Journal Info:
Nlm Unique ID:  101571433     Medline TA:  J Pathog     Country:  Egypt    
Other Details:
Languages:  eng     Pagination:  781652     Citation Subset:  -    
Lethbridge Laboratory, National Centres for Animal Disease, Canadian Food Inspection Agency, P.O. Box 640, Township Road 9-1, Lethbridge, AB, Canada T1J 3Z4.
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