| Rapid DNA haplotyping using a multiplex heteroduplex approach: application to Duchenne muscular dystrophy carrier testing. | |
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MedLine Citation:
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PMID: 7599638 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A new strategy has been developed for rapid haplotype analysis based on an initial multiplex amplification of several polymorphic sites, followed by heteroduplex detection. Heteroduplexes formed between two different alleles are detected because they migrate differently than the corresponding homoduplexes in Hydrolink-MDE gel. This simple, rapid method does not depend on specific sequences such as restriction enzyme sites or CA boxes and does not require the use of isotope. This approach has been tested using commonly occurring polymorphisms spanning the dystrophin gene as a model. We describe the use of the method to assign the carrier status of females in Duchenne muscular dystrophy (DMD) pedigrees. The method may be used for other genetic diseases when mutations are unknown or there are few dinucleotide markers in the gene proximity, and for the identification of haplotype backgrounds of mutant alleles. |
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Authors:
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T W Prior; G D Wenger; A C Papp; P J Snyder; M S Sedra; C Bartolo; J W Moore; W E Highsmith |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Human mutation Volume: 5 ISSN: 1059-7794 ISO Abbreviation: Hum. Mutat. Publication Date: 1995 |
Date Detail:
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Created Date: 1995-08-10 Completed Date: 1995-08-10 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9215429 Medline TA: Hum Mutat Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 263-8 Citation Subset: IM |
Affiliation:
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Department of Pathology, Ohio State University, Columbus 43210, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence DNA / analysis* Dystrophin / genetics* Electrophoresis, Polyacrylamide Gel Exons Female Haplotypes Heterozygote Detection / methods* Humans Molecular Sequence Data Muscular Dystrophies / genetics* Nucleic Acid Heteroduplexes / genetics* Pedigree Polymerase Chain Reaction Polymorphism, Genetic |
| Chemical | |
Reg. No./Substance:
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0/Dystrophin; 0/Nucleic Acid Heteroduplexes; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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