Document Detail

Rapid 3D printing of anatomically accurate and mechanically heterogeneous aortic valve hydrogel scaffolds.
MedLine Citation:
PMID:  22914604     Owner:  NLM     Status:  MEDLINE    
The aortic valve exhibits complex three-dimensional (3D) anatomy and heterogeneity essential for the long-term efficient biomechanical function. These are, however, challenging to mimic in de novo engineered living tissue valve strategies. We present a novel simultaneous 3D printing/photocrosslinking technique for rapidly engineering complex, heterogeneous aortic valve scaffolds. Native anatomic and axisymmetric aortic valve geometries (root wall and tri-leaflets) with 12-22 mm inner diameters (ID) were 3D printed with poly-ethylene glycol-diacrylate (PEG-DA) hydrogels (700 or 8000 MW) supplemented with alginate. 3D printing geometric accuracy was quantified and compared using Micro-CT. Porcine aortic valve interstitial cells (PAVIC) seeded scaffolds were cultured for up to 21 days. Results showed that blended PEG-DA scaffolds could achieve over tenfold range in elastic modulus (5.3±0.9 to 74.6±1.5 kPa). 3D printing times for valve conduits with mechanically contrasting hydrogels were optimized to 14 to 45 min, increasing linearly with conduit diameter. Larger printed valves had greater shape fidelity (93.3±2.6, 85.1±2.0 and 73.3±5.2% for 22, 17 and 12 mm ID porcine valves; 89.1±4.0, 84.1±5.6 and 66.6±5.2% for simplified valves). PAVIC seeded scaffolds maintained near 100% viability over 21 days. These results demonstrate that 3D hydrogel printing with controlled photocrosslinking can rapidly fabricate anatomical heterogeneous valve conduits that support cell engraftment.
L A Hockaday; K H Kang; N W Colangelo; P Y C Cheung; B Duan; E Malone; J Wu; L N Girardi; L J Bonassar; H Lipson; C C Chu; J T Butcher
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-08-23
Journal Detail:
Title:  Biofabrication     Volume:  4     ISSN:  1758-5090     ISO Abbreviation:  Biofabrication     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-08-24     Completed Date:  2013-01-04     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  101521964     Medline TA:  Biofabrication     Country:  England    
Other Details:
Languages:  eng     Pagination:  035005     Citation Subset:  IM    
Department of Biomedical Engineering, Cornell University, Ithaca, NY, USA.
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MeSH Terms
Alginates / chemistry
Aortic Valve / anatomy & histology*,  cytology
Biocompatible Materials / chemistry
Cell Survival
Cells, Cultured
Elastic Modulus
Glucuronic Acid / chemistry
Hexuronic Acids / chemistry
Hydrogel / chemistry*
Polyethylene Glycols / chemistry
Tissue Engineering
Tissue Scaffolds / veterinary*
Grant Support
Reg. No./Substance:
0/Alginates; 0/Biocompatible Materials; 0/Hexuronic Acids; 0/Polyethylene Glycols; 0/poly(ethylene glycol)diacrylate; 25852-47-5/Hydrogel; 576-37-4/Glucuronic Acid; 9005-32-7/alginic acid

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